Effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts |
| |
| Authors: | REN Yu-sheng WU Zong-gui CUI Fang JIA Guo-liang YU Shi-qing TANG Chao-wu LI Bo |
| |
| Institution: | 1. Department of Cardiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;
2. Xijing Hospital, Fourth Military Medical university, Xi'an 710032, China |
| |
| Abstract: | AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using 3H]-TdR incorporation and 3H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts. |
| |
| Keywords: | Platelet-derived growth factor Fibroblasts DNA Collagen synthesis |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
