Role of cyclic nucleotides in the acute hypoxic responses of hypoxic subcultured porcine pulmonary arterial smooth muscle and endothelial cells |
| |
| Authors: | ZENG Qing JIN Xian-rong WANG Di-xun HONG Zhi-gang HAO Tian-ling XIONG Zong-bin SUN Bing-yong |
| |
| Institution: | 1. Department of Pathophysiology,Tongji Medical University,Wuhan 430030,China;
2. epartment of Pathophysiology,Guilin Medical College, Guilin 541001,China;
3. Third Military Medical University, Chongqing 400038, China |
| |
| Abstract: | AIM: To detect the role of cyclic nucleotides in the alleviation of hypoxic pulmonary vasoconstriction (HPV) in chronic hypoxic animals. METHODS: The intracellular cAMP and cGMP of the cultured porcine pulmonary arterial smooth muscle cells (PASMC) and endothelial cells (PAEC) were assayed by RIA. The length of single PASMC during acute hypoxia was measured by imaging analysis system. RESULTS: The basal levels of cAMP and cGMP in PASMC and cGMP in PAEC of Chronic hypoxic groups decreased remarkably compared with normoxic groups (P＜0.01). Under acute hypoxia, the contents of cAMP and cGMP in PASMC of chronic hypoxic groups increased significantly (P＜0.01). Meanwhile, the percentage of PASMC with weak constrictive response in chronic hypoxic group was higher than that of control group. CONCLUSION:It's suggested that the changes of cAMP and cGMP in chronic hypoxic PASMC and PAEC might contribute to the increase in the basic tension of pulmonary artery and the alleviation of HPV in chronic hypoxic animals. |
| |
| Keywords: | Nucleotides cyclic Cells Anoxia Pulmonary artery Endothelium Muscle smooth |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
