Effect of formononetin on viability,migration and invasion of ovarian cancer cells in vitro |
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| Authors: | GU Cai-ru LIN Meng-yuan LIU Wei |
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| Institution: | 1.Department of Gynecology, Guangzhou Red Cross Hospital, Jinan University, Guangzhou 510220, China;2.Department of Gynecology, Shenzhen People's Hospital, Shenzhen 518020, China;3.Department of Breast, Guangzhou Red Cross Hospital, Jinan University, Guangzhou 510220, China. E-mail: lwei7@mail2.sysu.edu.cn |
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| Abstract: | AIM To observe the effect of formononetin on the viability, migration and invasion of ovarian cancer cells, and to explore its mechanism. METHODS Human ovarian serous cystadenocarcinoma SKOV-3 cells were cultured in vitro. The cells were treated with formononetin at 0， 25, 50 and 100 μmol/L for 48 h. The cell viability was measured by MTS assay. The migration and invasion abilities of the SKOV-3 cells were detected by scratch wound assay and Transwell assay. RT-qPCR and Western blot were used to detect the mRNA and protein levels of E-cadherin and matrix metalloproteinase-9 (MMP-9). RESULTS The viability of SKOV-3 cells was decreased with the increase in the formononetin concentration compared with control group (P<0.01). The wound migration distance of the cells in 50 μmol/L formononetin group was less than that in control group (P<0.01). The number of invasive SKOV-3 cells across the Transwell sub-compartment was significantly decreased in 50 μmol/L formononetin group compared with control group (P<0.01). The mRNA and protein levels of E-cadherin in 50 μmol/L formononetin group were significantly higher than those in control group (P<0.01), while the mRNA and protein levels of MMP-9 in 50 μmol/L formononetin group were significantly lower than those in control group (P<0.01). CONCLUSION Formononetin inhibits the migration and invasion abilities of ovarian cancer SKOV-3 cells by increasing expression of E-cadherin and decreasing expression of MMP-9. |
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| Keywords: | Ovarian cancer Formononetin Cell migration Cell invasion E-cadherin Matrix metalloproteinase-9 |
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