A method of inducing mouse embryonic stem cells cultured in vitro to differentiate into cardiomyocytes |
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| Authors: | ZENG Qiu-tang CAO Lin-sheng ZHU Wu-qiang |
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| Institution: | Institute of Cardiovascular Diseases, Tongji Medical College, Hua Zhong Science and Technology University, Wuhan 430022, China |
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| Abstract: | AIM:To evaluate the method of inducing mouse embryonic stem (ES) cells in vitro to differentiate into cardiomyocytes without using any chemical reagents.METHODS:BRL conditioned medium was used to promote the growth of ES cells and maintain them in an undifferentiated state before the experiment of differentiation. Then a three-step method including ES cell culture in hanging drops and in suspending was used to induce the differentiation of ES cells. RESULTS:Rhythmically contracting cells were observed among differentiated cells, which were proved to be cardiomyocytes with electron microscope and immunocytochemistry. CONCLUSION:A simple and economical method was established to induce mouse ES cells cultured in vitro to differentiate into cardiomyocytes without using any chemical reagents. |
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| Keywords: | Embryo Stem cells Myocardium Cells |
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