The effect of EBV latent membrane protein 1 on proliferation and cell cycle of nasopharyngeal carcinoma cells |
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| Authors: | HUANG Pei-chun LIANG Hong-ying DENG Hui-hua CAI Kang-rong ZHAO Ming-lun |
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| Institution: | Department of Pathophysiology, Guangdong Medical College, Zhanjiang 524023, China |
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| Abstract: | AIM:To investigate the effect of Epstain-Barr virus latent membrane protein 1 (EBV-LMP1) on proliferation and cell cycle of nasopharyngeal carcinoma (NPC) cells. METHODS:The expression of EBV-LMP1 was detected by immunohistochemical method (LSAB). Proliferation of NPC cells was identified by MTT method. Cell cycle percentage was detected by flow cytometry analysis. RESULTS: OD value of EBV-LMP1 expressive NPC cells L-CEN1 was much higher than that of both EBV-LMP1 negative NPC cells V-CEN1 and CNE1 (P＜0.01). Compared with the cell cycle percentage in both V-CNE1 and CNE1, the percentage of G1 was significantly decreased and the percentage of S was much increased in L-CNE1 (P＜0.01). But no obvious differences were observed in all cell cycle percentage between V-CNE1 and CNE1 (P＞0.05).CONCLUSION:The expression of EBV-LMP1 on NPC cell might cause some change of cell cycle and enhance cell proliferation. |
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| Keywords: | Epstein-Barr virus Nasopharyngeal neoplasms Cell line Cell division Cell cycle |
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