Effect of nitric oxide and peroxynitrite on lung injury induced by ischemia-reperfusion of hind limbs in rats |
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| Authors: | ZHOU Jun-lin LING Yi- ling LI Chen-li GU Zhen-yong SHI Zhong-li DING Chun-hua |
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| Institution: | Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China |
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| Abstract: | AIM:To observe the changes in nitric oxide(NO) and peroxynitrite anion (ONOO- ) in the injuried lung following the ischemia-reperfusion of hind limbs and evaluate the contribution of NO and ONOO- to tissue injury.METHODS:A model of hind limbs ischemia was made by clamping infrarenal aorta with a microvascular clip and lung injury occurring after reperfusion.Lung t issue was obtained from the animals received sham operation(group 1),4 hours ischemia without reperfusion(group2),1 hour reperfusion following 4 hours ischemia(group3)and 4 hours reperfusion fol owing 4 hours ischemia(group4).The contents of MDA,NO2-/NO3- and the activities of SOD in the lung were examined.Immunohistochemical echnique was used to determine the immunoreactivity to iNOS and nitrotyrosine(NT)-a specific "footprint" of peroxynitrite.RESULTS:Compared with group1 and group2,the contents of MDA and NO2-/NO3- increased significantly (P<0.05) and the activities of SOD decreased markedly(P＜0.05) in group3 and group4.Immunohistochemical examination demonstrated intense staining for iNOS and NT throughout the lung in group3 and group4.CONCLUSION:NO and ONOO- are involved in oxidant-mediated lung injury following reperfusion of ischemic hind limbs. |
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| Keywords: | Nitric oxide Anions Extremities Lung Reperfusion injury |
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