Expression of calcium handling protein in diastolic heart failure |
| |
| Authors: | ZHONG Ming ZHANG Yun ZHANG Wei BIAN Ji-feng GENG Zhao ZHAO Jing |
| |
| Institution: | 1. Department of Senior Health Care;
2. Department of Cardiology, the Affiliated Hospital of Shandong Medical University, ;
3. Department of Molecular Biology of Shandong Medical University, Jinan 250012, China |
| |
| Abstract: | AIM:To elucidate molecular mechanisms underlying calcium handling protein in diastolic heart failure (DHF) from mRNA level and protein expression, including calcium adenosine triphosphatase (Ca2+-ATPase), phospholamban, ryanodine receptor, calsequestrin and L-type calcium channel.METHODS:The mRNA of these calcium handling genes were detected by RT-PCR, and the protein levels were analyzed by Western blot analysis.RESULTS:Compared with sham-operated rabbits, the steady-state levels of mRNA encoding the SR Ca2+-ATPase and cardiac L-type calcium channel were decreased significantly in rabbits with DHF, and protein level of SR Ca2+-ATPase was greatly reduced, whereas the mRNA and protein levels of other calcium handling protein were unchanged.CONCLUSION:L-type calcium channel and the sarcoplasmic reticular Ca2+-ATPase were down regulated in DHF. These changes may be a contributory factor for DHF. |
| |
| Keywords: | Heart failure Calmodulin Gene expression |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|
