Expression of bFGF mRNA in pulmonary artery of rat with chronic hypoxia and its effect on tension of rat pulmonary artery in vitro |
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| Authors: | RAN Pi-xin NIE Sheng-li CHEN Yan-fang CHEN Shun-cun |
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| Institution: | 1. Guangzhou Institute of Respiratory Disease, Guangzhou Medical College, Guangzhou 510182, China;
2. The Second Affiliated Hospital, Sun Yat-sen University of Medical Sciences;
3. Guangdong Provincial Hospital |
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| Abstract: | AIM: To explore the role of basic-fibroblast growth factor (bFGF) in the development of pulmonary hypertension induced by hypoxia. METHODS: 1) The pulmonary arteries of SD rats with hypoxia for one and two weeks were isolated, from which the total RNA were extracted by acid guanidinium thiocyanate-phenol-chlorform .Then the levels of mRNA were measured by RT-PCR. 2) About 3mm-long arterial rings cut from SD rat pulmonary arterial stem were suspended between stainless steelhooks in chamber with warmed (37℃) Kreb's solution. Different concentrations of bFGF were added in a cumulative fashion into the chamber where the rings were suspended. The cumulative concentration response curve was obtained. RESULTS: 1)The levels of bFGF mRNA in pulmonary artery of rats with hypoxia were increased significantly compared with those that without hypoxia (2578±384 counts·min-1 (control) vs 5303±756 (hypoxia) for 1 week and 4054±547 (hypoxia) for 2 weeks, P all ＜0.05). 2) bFGF at concentrations ranged from 5.56×10-10~2.78×10-7mol/L caused dose-dependent contraction of vessel rings of rat pulmonary artery (r=0.695,P＜0.05), with EC50 being 2.62×10-7mol/L. CONCLUSION: bFGF may play an important role in the hypoxic pulmonary hypertension. |
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| Keywords: | Fibroblast growth factor basic Pulmonary artery Rats Anoxia |
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