The effect of human complement C5b~9 complex on nitric oxide synthesis in glomerular mesangial cells of rats |
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| Authors: | WANG Ying-wei HE Qiu-zao QIN Hui-lian TANG Ren-xian GAO Feng-guang ZHANG Guang-yi |
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| Institution: | Dept of Immunology, Xuzhou Medical College, Xuzhou 221002, China |
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| Abstract: | AIM:To study the effect of human complement C5b~9 complex on nitric oxide(NO) synthesis of glomerular mesangial cells (MC). METHODS: First, the human complement C5b~9 complexes were isolated and glomerular MC of rats were cultured. Second, the MC were stimulated with C5b~9 complex and changes of metabolism products of NO(NO3- and NO2) in MC culture supernatant at 6,24 and 48 hours after C+5b~9 stimulating were detected. Moreover, cGMP levels in cultured MC were also measured. RESULTS: NO3-／NO2- contents from culture supernatant and cGMP levels in MC were increased parallelly after C5b~9 complex stimulation. Further, NO synthesis was inhibited by L-NG-nitro-arginine-methylester(L-NAME). CONCLUSION: NO synthesis of rat glomerular MC was incerased by human complement C5b~9 stimulation. |
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