| 阿魏酸抗LPS诱导的奶牛子宫内膜上皮细胞炎性作用 |
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| 引用本文： | 张志聪,吕安,宫平,张彤,刘艺林,李德琰,姜晓茜,刘蓓桦,刘凤华.阿魏酸抗LPS诱导的奶牛子宫内膜上皮细胞炎性作用[J].北京农学院学报,2016(4):56-60. |
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| 作者姓名： | 张志聪 吕安 宫平 张彤 刘艺林 李德琰 姜晓茜 刘蓓桦 刘凤华 |
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| 作者单位： | 北京农学院动物科学技术学院,北京,102206 |
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| 基金项目： | 国家“十二五”科技支撑计划重点项目(2011BAD34B01) |
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| 摘 要： | 目的]为了探明阿魏酸的抗炎机制.方法]采用组织块培养法获得奶牛子宫内膜上皮细胞.用噻唑蓝(MTT法)检测细胞活力.采用荧光定量PCR方法,分别对对照组、模型组、药物作用高、中、低剂量组在LPS作用3h、6h炎症基因IL-1β、IL-6、IL-8和TNF-α的mRNA变化进行检测.结果]阿魏酸在一定浓度范围内对细胞增殖无显著影响.药物预处理细胞能够显著降低LPS诱导的IL-1β、IL-6、IL-8和TNF-α的mRNA表达.结论]阿魏酸能够显著降低LPS刺激细胞后炎症细胞因子的表达,说明阿魏酸是通过抑制炎症因子的表达发挥其抗炎作用.
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| 关 键 词： | 阿魏酸 奶牛子宫内膜上皮细胞 细胞因子 脂多糖 抗炎作用 |
Anti-inflammatory effect of ferulic acid on the bovine endometrial epithelial cell treated by LPS |
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| ZHANG Zhicong,L An,GONG Ping,ZHANG Tong,LIU Yilin,LI Deyan,JIANG Xiaoqian,LIU Beihua,LIU Fenghua.Anti-inflammatory effect of ferulic acid on the bovine endometrial epithelial cell treated by LPS[J].Journal of Beijing Agricultural College,2016(4):56-60. |
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| Authors: | ZHANG Zhicong L An GONG Ping ZHANG Tong LIU Yilin LI Deyan JIANG Xiaoqian LIU Beihua LIU Fenghua |
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| Institution: | ZHANG Zhicong,L(U) An,GONG Ping,ZHANG Tong,LIU Yilin,LI Deyan,JIANG Xiaoqian,LIU Beihua,LIU Fenghua |
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| Abstract: | Objective]The aim of this study was to explore the anti-inflammatory mecchanism of ferulic acid.Methods] Bovine endometrial epithelial cells (BEEC)were cultured and divided into control group,model group,low,medium,and high LPS groups.The cell viability of BEEC was tested by MTT.RT-PCR was used to detect cytokines (IL-1β,IL-6,IL-8,and TNF-α)mRNA expression of BEEC induced by LPS after 3 h and 6 h.Result]There were no significant effects on cell viability among gourps.Ferulic acid significantly reduced IL-1β,IL-6,IL-8,and TNF-α mRNA expression induced by LPS.Conclusion] Ferulic acid can decrease significantly the cytokines expression of BEEC treated with LPS,showing anti-inflammation effect. |
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| Keywords: | ferulic acid bovine endometrial epithelial cell cytokines Lipopolysaccharide anti-inflammatory |
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