| 草莓灰霉病菌的培养、毒素提取及生物测定 |
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| 引用本文： | 朱宝成,周毓君.草莓灰霉病菌的培养、毒素提取及生物测定[J].植物病理学报,1994,24(3):239-243. |
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| 作者姓名： | 朱宝成 周毓君 |
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| 作者单位： | 河北大学生物系, 保定 071002 |
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| 摘 要： | 草莓灰霉病菌(Botrytis cinerea Persoon)在25℃、pH3~4的Peberdy培养基中生长良好,并可产生引起草莓愈伤组织细胞死亡的毒素。产生毒素的高峰期为静置培养20天。灰霉菌发酵液中的毒素可用氯仿进行提取,经提取后的毒素液在269nm处有吸收峰。本研究建立了利用草莓愈伤组织细胞的荧光活性测定毒力的方法。
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| 关 键 词： | 草莓 灰霉病菌 毒素 |
THE CULTURE, ISOLATION AND BIOASSAY OF BC-TOXIN OF BOTRYTIS CINEREA PERSOON |
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| Zhu Baocheng Liang Bofan Zhou Yujun Guo Mingshen.THE CULTURE, ISOLATION AND BIOASSAY OF BC-TOXIN OF BOTRYTIS CINEREA PERSOON[J].Acta Phytopathologica Sinica,1994,24(3):239-243. |
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| Authors: | Zhu Baocheng Liang Bofan Zhou Yujun Guo Mingshen |
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| Institution: | Dept. of Biology, Hebei University, Baoding 071001 |
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| Abstract: | Botrytis cinerea Persoon isolated from Fraguria ananassa was grown well in Peberdy's liquid medium(pH3~4) at 25℃ and produced toxin which inhibits the cell activity of the callus of Fraguria ananassa. The highest toxicity of culture filtrates occurred on the twentieth day after incubation. The crude toxin was obtained from condensed culture filtrates by extraction with chloroform. The toxin exhibited absorption maximum at 269nm.
A bioassay method via cell fluorescence activity of the callus is established. |
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| Keywords: | Strawberries Botrytis cinerea Toxin |
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