Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in cow uteri with adenomyosis during follicular phase |
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| Authors: | Lio Moreira Eulógio Carlos Queiroz de Carvalho Maria Clara Caldas-Bussiere |
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| Institution: | Laboratório de Sanidade Animal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Hospital Veterinário-Av. Alberto Lamego, 2000, Parque Califórnia, 28013-602, Campos dos Goytacazes, RJ, Brazil. lio_moreira@hotmail.com |
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| Abstract: | The present investigation was intended to show a different immunohistochemical profile of matrix metalloproteinase-2 and Tissue
inhibitor metalloproteinase-2 in bovine uteri with adenomyosis during follicular phase. Uterine samples of 32 cows in reproductive
age were taken from the medial third of one of the uterine horns and grouped according to the adenomyosis degree (superficial
and deep). Tissue sections (4 μm) were incubated overnight at 4°C with monoclonal antibody for matrix metalloproteinase-2
and Tissue inhibitor metalloproteinase-2. Staining intensities were evaluated in the luminal epithelium, ectopic and dystopic
endometrial tissue (stroma, capillaries and glands), endometrial-myometrial border, myometrium, myometrial vessels (middle
tunic and endothelium). The matrix metalloproteinase-2 expression was higher for deep adenomyosis samples, showing a differential
mean reactivity in superficial endometrium, myometrial vessels, myometrium adjacent to adenomyotic focus and endometrial-myometrial
border (P < 0.05). Moreover, matrix metalloproteinase-2 expression was higher in deep adenomyosis samples than that of Tissue inhibitor
metalloproteinase-2 in almost all uterine structures analyzed (except for the endometrial and myometrial vessels and endometrial-myometrial
border). The opposite was observed in the follicular phase, for both normal specimens and with superficial adenomyosis, where
Tissue inhibitor metalloproteinase-2 expression was higher than that of matrix metalloproteinase-2. In conclusion, a differential
pattern of matrix metalloproteinase-2 and Tissue inhibitor metalloproteinase-2 was observed in cow uteri with adenomyosis. |
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