| 15株鱼源致病性鲫鱼诺卡氏菌的聚类分析 |
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| 引用本文： | 满其蒙,冯娟,区又君,苏佩亭,徐力文.15株鱼源致病性鲫鱼诺卡氏菌的聚类分析[J].南方水产,2013(5):86-92. |
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| 作者姓名： | 满其蒙 冯娟 区又君 苏佩亭 徐力文 |
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| 作者单位： | [1]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]广州大学生命科学学院,广东广州510006 |
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| 基金项目： | 广东省科技计划项目（2011B020307003）;中囤水产科学研究院级基本科研业务费项目（2012A0503,2013A0604） |
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| 摘 要： | 文章对2006年～2012年从华南地区采样或送检的患“结节病”的鱼类（8个鱼种）样品中分离到的15个病原菌株进行了系统发育和聚类分析。系统发育分析结果显示，所有供试菌的16SrDNA序列均与鲫鱼诺卡氏菌（Nocardia seriolae）聚为一支，同源性为99％，且明显地分为2个类群；聚类结果显示，15个菌株在T=23时可分为2个组群，在T=11时2个组群，分别可分为2个和4个亚群。研究结果显示15株鲫鱼诺卡氏菌存在多样性。
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| 关 键 词： | 鲫鱼诺卡氏菌 病原 系统发育分析 聚类分析 |
Cluster analysis on 15 strains of pathogenic Nocardia seriolae from fish |
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| MAN Qimeng,FENG Juan,OU Youjunj,SU Peiting,XU Liwen.Cluster analysis on 15 strains of pathogenic Nocardia seriolae from fish[J].South China Fisheries Science,2013(5):86-92. |
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| Authors: | MAN Qimeng FENG Juan OU Youjunj SU Peiting XU Liwen |
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| Institution: | j ( I. Key Lab. of South China Sea Fishery Resources Exploitation ＆ Utilization, Ministry of Agriculture ; South China Sea Fisheries Research Institute, Chinese Academy of Fishery＂ Sciences, Guangzhou 510300, China; 2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China; 3. School of Life Sciences of Guangzhou University, Guangzhou S10006, China) |
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| Abstract: | Using phylogenetic and cluster analysis, we studied 15 strains of bacteria isolated from eight speeies of South China san]- piing and inspected fish that were suffering from sarcoidosis. Phylogenetic analysis indicates that the 16S rDNA sequences of 15 isolated strains, which share 99% similarity to that of Nocardia seriolae, have a close evolutionary relationship with the member of the N. seriolae, and could be grouped into 2 clusers. Cluster analysis shows that 15 isolated strains could be divided into two groups when T = 23, which could be divided into two and four subgroups respectively when T = 11. The results suggest that diversity exists in the 15 strains of N. seriolae. |
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| Keywords: | Nocardia seriolae pathogen phylogenetic analysis cluster analysis |
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