| 陇东野生紫花苜蓿的遗传特异性分析 |
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| 引用本文： | 张雪婷,师尚礼.陇东野生紫花苜蓿的遗传特异性分析[J].草地学报,2009,17(3):343-348. |
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| 作者姓名： | 张雪婷 师尚礼 |
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| 作者单位： | 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃, 兰州, 730070 |
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| 基金项目： | 国家科技支撑计划，农业部公益性行业(农业)科研专项基金 |
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| 摘 要： | 为初步判定陇东野生紫花苜蓿(Native Medicago sativa L. cv. Longdong)的遗传特异性,及与其他苜蓿材料的亲缘关系,借助垂直板聚丙烯酰胺凝胶电泳技术,对包括该材料在内的41个紫花苜蓿材料进行POD同工酶酶谱特征分析。结果表明:供试材料共出现22条酶带,最多9条,最少5条;陇东野生紫花苜蓿出现6条酶带,其特征谱带为Rf0.26;将0,1二态性矩阵转化为遗传距离矩阵进行聚类,陇东野生紫花苜蓿单独聚为一类。聚类分析的结果表明,陇东野生紫花苜蓿与其他40个材料存在较大的遗传差异,为其在形态学上与其他苜蓿的差异提供了有利支持。
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| 关 键 词： | 陇东野生紫花苜蓿 POD同工酶 酶谱分析 特异性 聚类分析 |
| 收稿时间： | 2008-09-10 |
Genetic specificity in Native Medicago sativa L. cv. Longdong |
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| ZHANG Xue-ting,SHI Shang-li.Genetic specificity in Native Medicago sativa L. cv. Longdong[J].Acta Agrestia Sinica,2009,17(3):343-348. |
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| Authors: | ZHANG Xue-ting SHI Shang-li |
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| Institution: | Pratacultural College, Gansu Agricultural University;Key Laboratory of Grassland Ecosystem of Ministry of Education; Sino-U.S.Centers for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China |
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| Abstract: | For analyzing the genetic specificity of Longdong native alfalfa(Medicago sativa L.cv.Longdong) and estimating the genetic relationship between it and other alfalfa cultivars,the POD isozyme of 41 alfalfa cultivars including Longdong native cultivar were analyzed using the polylamide gel eletrophoresis technique.The results show that 22 enzyme bands in total appeared from the experimental materials with the largest number of enzymes at 9 and the least at 5.Native Longdong alfalfa had 6 enzyme bands and the ... |
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| Keywords: | Longdong native alfalfa(Medicago sativa L cv Longdong) Peroxidase(POD) isozyme Zymogram analysis Specificity Cluster analysis |
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