| 一种用于口蹄疫病毒实时荧光RT-PCR检测的引物和方法 |
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| 引用本文： | 邱杨,黄伟,刘建丽,赵丽.一种用于口蹄疫病毒实时荧光RT-PCR检测的引物和方法[J].动物检疫,2012(1):34-36. |
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| 作者姓名： | 邱杨 黄伟 刘建丽 赵丽 |
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| 作者单位： | 东莞出入境检验检疫局,东莞523072 |
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| 基金项目： | 质检公益性行业科研专项项目（201110036） |
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| 摘 要： | 本文建立了一种用于口蹄疫病毒实时荧光RT-PCR检测的引物和方法。分析所有已报道的口蹄疫病毒通用型基因组序列,分别设计引物和荧光探针,通过实时荧光RT-PCR检测,得到口蹄疫通用型的特异性荧光曲线,可以确定血清型,检测结果准确、可靠。
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| 关 键 词： | 口蹄疫病毒 引物 RT-PCR |
A Set of Primers and the Method of Real-time RT-PCR for FMDV Detection |
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| Authors: | Qiu Yang Huang Wei Liu Jianli Zhao Li |
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| Institution: | ,Chen Jinhui(Dongguan Entry-Exit Inspection and Quarantine Bureau of the People＇s Republic of China,Dongguan,Guangdong,523072) |
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| Abstract: | Primers and the method of Real-time RT-PCR for FMDV were developed.All the reported genome sequences of FMDV were analysed and the primers and fluorescence probes were designed.By the Real-time RT-PCR detection,a specific fluorescence curve was got.The real-time RT-PCR could be used for confirmation of FMDV serotypes with sound and reliable results. |
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| Keywords: | FMDV primer real-time PCR |
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