| 非洲猪瘟病毒检测试剂盒的研制 |
| |
| 引用本文： | 董志珍,赵祥平,张霞,肖妍,栾慎顺.非洲猪瘟病毒检测试剂盒的研制[J].动物检疫,2012(1):31-33. |
| |
| 作者姓名： | 董志珍 赵祥平 张霞 肖妍 栾慎顺 |
| |
| 作者单位： | 天津出入境检验检疫局,天津300461 |
| |
| 基金项目： | 进出境重大动物疫病预警、检测和处理技术研究（2006BAD06A14）资助 |
| |
| 摘 要： | 针对非洲猪瘟病毒（ASFV）P54基因建立荧光PCR检测方法,通过优化引物、TaqMan探针浓度及反应温度,研发了ASFV检测的荧光PCR检测试剂盒。经灵敏度的重复性试验及3种不同型号荧光PCR仪使用验证,所研发的试剂盒灵敏度为101copy/μL,适用于不同型号荧光PCR仪使用,可用于对非洲猪瘟病毒的快速检测。
|
| 关 键 词： | 非洲猪瘟病毒 荧光定量PCR 试剂盒 |
Development of African Swine Fever Virus Detection Kit |
| |
| Authors: | Dong Zhizhen Zhao Xiangping Zhang Xia Xiao Yan Luan Shenshun |
| |
| Institution: | (Tianjin Exit and Entry Inspection and Quarantime Bureau,Tianjin 300461) |
| |
| Abstract: | A fluorescence polymerase chain reaction（PCR）assay was developed with ASFV p54 gene for the detection of African swine fever virus.And the ASFV fluorescent quantitative polymerase chain reaction（PCR）test kit was obtained through optimization of the primer and the fluorescence labeled TaqMan probe concentration and reaction temperature.Several snsitivity tests and verification with three different instrument models showed that,the sensitivity of kit was 101 copy/μL and suitable for different types of instrument.All these proved that the kit could be used for fast detection of ASFV. |
| |
| Keywords: | African swine fever virus Real-time RT-PCR Test kit |
| 本文献已被 维普 等数据库收录！ |
|
