检测动物产品中水貂阿留申病毒和犬细小病毒复合PCR方法的建立和应用 |
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引用本文: | 杜雄伟,李叶,胡传伟.检测动物产品中水貂阿留申病毒和犬细小病毒复合PCR方法的建立和应用[J].动物检疫,2012(8):43-45. |
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作者姓名: | 杜雄伟 李叶 胡传伟 |
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作者单位: | [1]大连民族学院生命科学学院,大连16600 [2]辽宁出入境检验检疫局,大连116001 |
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基金项目: | 中央高校自主基金项目(DC12010304) |
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摘 要: | 水貂阿留申病(AD)和犬细小病毒病(CP)是危害经济动物健康的重要疫病,可造成巨大的经济损失。本研究建立了水貂阿留申病毒(ADV)、犬细小病毒(CPV)复合PCR检测方法,并对临床样品进行了大量检测,结果表明本方法特异、敏感、简便、快速,非常适宜临床样品的大量筛选检测,对经济动物疫情的快速诊断与控制、加强经济动物及其产品进出境的检验检疫工作,御疫情于国门之外有重要的意义。
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关 键 词: | 水貂阿留申病毒(ADV) 犬细小病毒(CPV) 复合PCR |
Establishment and Application of Multi-PCR Detective Methods for the Detection of Aleutian Mink Disease Virus and Canine Parvovirus in Animal Products |
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Authors: | Du Xiongwei Li Ye Hu Chuanwei |
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Institution: | 1 Dalian nationalities university, Dalian, 116600, China; 2 Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian, 116001, China) |
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Abstract: | Aleutian mink disease and canine parvovirus disease are two important diseases causing great economic loss to fur animal industry. In the study, a multi-PCR assay was developed for detection of Aleutian mink disease and canine parvovirus and the assay was applied in testing a lot of clinical samples. The results showed that the assay was specific, sensitive and rapid, and suitable for screening test. |
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Keywords: | Aleutian mink disease parvovirus canine parvovirus multi-PCR |
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