| 河北道地药材紫菀中总黄酮的荧光分析 |
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| 引用本文： | 冯俊霞,陆敏,郭瑞霞,戚秀菊.河北道地药材紫菀中总黄酮的荧光分析[J].湖北农业科学,2012,51(15):3323-3325,3329. |
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| 作者姓名： | 冯俊霞 陆敏 郭瑞霞 戚秀菊 |
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| 作者单位： | 1. 石家庄学院化工学院,石家庄,050035
2. 石家庄学院国有资产管理处,石家庄,050035 |
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| 基金项目： | 河北省教育厅科学研究计划项目(Z2010312) |
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| 摘 要： | 以槲皮素为标准品,用荧光分光光度法测定中药材紫菀(Aster tataricus L.f.)中总黄酮含量,该方法的检出限为2.51×10-7 g/L,线性回归方程为y=531.840 48x-3.544 48,相关系数R2=0.999 03,线性范围为6.7×10-6～6.0×10-4 g/L；紫菀中总黄酮的平均含量为4.896×10-3g/L,样品测定的RSD为0.92％,平均回收率为93.19％.该方法操作简便、准确度高,可以为中药材紫菀质量控制提供参考.
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| 关 键 词： | 紫菀(Aster tataricus L.f.) 总黄酮 荧光分光光度法 |
Determination of Total Flavanoid in Radix Aster from Hebei Province by Spectrofluorimetry |
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| FENG Jun-xia
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LU Min
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GUO Rui-xia
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QI Xiu-ju.Determination of Total Flavanoid in Radix Aster from Hebei Province by Spectrofluorimetry[J].Hubei Agricultural Sciences,2012,51(15):3323-3325,3329. |
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| Authors: | FENG Jun-xia LU Min GUO Rui-xia QI Xiu-ju |
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| Institution: | b(Shijiazhuang University,a.School of Chemical Engineering;b.Adminstative Office of State Owned Assets,Shijiazhuang 050035,China) |
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| Abstract: | Quercetin was used as standard material to establish a new method for determining total flavanoid in Aster tataricus L.f.The detection limit of this method was 2.51×10-7 g/L,and the linear range was between 6.7×10-6～6.0×10-4 g/L,and the equation of linear regression was y=531.840 48x-3.544 48,and correlation coefficient R2 was 0.999 03.The average amount of total flavanoid in A.tataricus was 4.896×10-3 g/L,and RSD was 0.92%,the average recovery was 93.19%.It is a simple and accurate method to determine total flavanoid in A.tataricus with spectrofluorimetry.The new method can be used for quality control of Chinese medicinal plant A.tataricus. |
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| Keywords: | radix Aster(Aster tataricus L f ) total flavanoid spectrofluorimetry |
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