| 猪场蚊子中乙型脑炎病毒的RT-PCR检测 |
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| 引用本文： | 魏燕鸣.猪场蚊子中乙型脑炎病毒的RT-PCR检测[J].湖北农业科学,2012,51(14):3104-3106. |
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| 作者姓名： | 魏燕鸣 |
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| 作者单位： | 华中农业大学动物医学院,武汉,430070 |
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| 基金项目： | 国家公益性行业(农业)科研专项(201203082) |
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| 摘 要： | 收集湖北省与安徽省部分猪场的蚊子样本,用RT-PCR检测蚊子样本是否携带有乙型脑炎病毒,并对猪群乙型脑炎病毒抗体进行了检测.结果表明,53份蚊子样品中乙型脑炎病毒阳性样品为44份,表明这些猪场的蚊子广泛携带有乙型脑炎病毒.从阳性PCR产物中随机选出8个,并将其克隆到pEASY-T1载体中,得到5个阳性重组质粒,进行序列测定和进化树分析,结果表明阳性蚊子样本携带的都是基因Ⅲ型乙型脑炎病毒.猪场的1 401份血清样品的乙型脑炎病毒抗体阳性率为57.7％,说明这些猪场猪群的乙型脑炎病毒感染率较高.
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| 关 键 词： | 猪场 蚊子 乙型脑炎病毒 RT-PCR |
Detection of Encephalitis Virus in Mosquitoes from Pig Farms by RT-PCR |
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| WEI Yan-ming.Detection of Encephalitis Virus in Mosquitoes from Pig Farms by RT-PCR[J].Hubei Agricultural Sciences,2012,51(14):3104-3106. |
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| Authors: | WEI Yan-ming |
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| Institution: | WEI Yan-ming(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | Mosquito samples were collected from pig farms in Hubei and Anhui province to test the existence of Japanese encephalitis virus(JEV) by RT-PCR.The results showed that 44 out of 53 samples were positive,illustrating that JEV was widely carried by mosquitoes in these pig farms.8 of the 44 positive PCR products were randomly selected and cloned into pEASY-T1 vector for sequencing and phylogenetic tree analysis;the results revealed that these samples were all genotype Ⅲ JEV.The positive rate of JEV in 1 401 serum samples in the pig farms was 57.7%,indicating a high infection rate of JEV in these farms. |
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| Keywords: | pig farms mosquito Japanese encephalitis virus RT-PCR |
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