| 松辽黑猪×野猪杂交猪H-FABP mRNA基因的组织分布研究 |
| |
| 引用本文： | 秦莹,高妍,张永宏,刘同欣,马倩,朱银莉,孙博兴,赵志辉,张嘉保.松辽黑猪×野猪杂交猪H-FABP mRNA基因的组织分布研究[J].安徽农业科学,2009,37(23):10902-10903. |
| |
| 作者姓名： | 秦莹 高妍 张永宏 刘同欣 马倩 朱银莉 孙博兴 赵志辉 张嘉保 |
| |
| 作者单位： | 吉林大学畜牧兽医学院,吉林长春,130062;吉林大学畜牧兽医学院,吉林长春,130062;吉林大学实验动物中心,吉林长春,130062;吉林大学实验动物中心,吉林长春,130062 |
| |
| 基金项目： | 国家高技术研究发展计划，吉林省科技厅项目 |
| |
| 摘 要： | 目的]研究松辽黑猪×野猪杂交猪H-FABP mRNA基因的组织分布，为探明该基因的生理功能提供依据。方法]采用RT-PCR技术获得松野杂交猪的cDNA，经PCR扩增后对目的片段进行电泳鉴定和测序，检测H-FABP mRNA在松辽黑猪×野猪杂交猪组织中的分布情况。结果]在选取的皮下脂肪、腹脂、乳腺、背最长肌、臂三头肌、心脏、肝脏、脾脏、肺脏、肾脏、回肠、十二指肠共12种组织中：H-FABP mRNA均有表达。结论]H-FABP基因在组织中广泛分布说明了其功能的重要性及多重性。
|
| 关 键 词： | 松辽黑猪×野猪杂交猪 H-FABP mRNA 组织分布 |
Distribution of H-FABP mRNA in Hybrids of Songliao Black Pig×Sus scrofa |
| |
| QIN Ying et al.Distribution of H-FABP mRNA in Hybrids of Songliao Black Pig×Sus scrofa[J].Journal of Anhui Agricultural Sciences,2009,37(23):10902-10903. |
| |
| Authors: | QIN Ying |
| |
| Institution: | QIN Ying et al(College of Animal Science , Veterinary Medicine,Jilin University,Changchun,Jilin 130062) |
| |
| Abstract: | Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig×Sus scrofa,so as to provide references for revealing the physiological functions of this gene.Method] With the mRNA from different tissues as template,RT-PCR amplification was carried out for cloning cDNA of hybrid pig,which was then used for PCR reaction using specific primers;the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig×Sus scrofa.Result] H-FABP expressed in all the twelve tissues including subcutaneous fat,abdominal fat,mammary gland,longissimus dorsi muscle,dorsal deltoid muscle,heart,liver,spleen,lung,kidney,ileum and duodenum.Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple. |
| |
| Keywords: | H-FABP mRNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
