| 麦麸中小麦矮腥黑穗病菌孢子的提取方法 |
| |
| 引用本文： | 章桂明,卢俭,田子章,陈枝楠,邓琼,李一农.麦麸中小麦矮腥黑穗病菌孢子的提取方法[J].植物病理学报,2002,32(4):319-325. |
| |
| 作者姓名： | 章桂明 卢俭 田子章 陈枝楠 邓琼 李一农 |
| |
| 作者单位： | 深圳出入境检验检疫局, 广东深圳 518010 |
| |
| 摘 要： | 对麦麸中小麦矮腥黑穗病菌孢子的提取方法进行了研究。通过建立的"过筛-α-淀粉酶降解"、"过筛-密度梯度离心"和"过筛-α-淀粉酶降解-密度梯度离心"等方法能从麦麸中提取TCK孢子。提取的TCK孢子数量与网筛、α-淀粉酶和密度梯度离心的应用情况有关,而提取的TCK孢子纯度则主要与密度梯度离心有关。选用双层纱布和5种不同规格的网筛或网筛组合进行过筛能弃除83.88%~94.09%的麦麸,应用"过筛-α-淀粉酶降解"方法能弃除95.01%~99.00%的麦麸,并能提取19.2%~51.7%的TCK孢子。在"过筛-密度梯度离心"实验中,只有60目+200目+300目+30μm+11μm该网筛组合处理能获取7.2%的纯孢子,密度梯度离心对孢子的网嵴高度、自发荧光和萌发没有影响;在"过筛-α-淀粉酶降解-密度梯度离心"实验中,60目+200目+300目和60目+200目+300目+30μm+11μm这2组网筛处理可分别得到18.8%和12.2%的纯孢子,该处理对孢子网嵴高度没有影响。
|
| 关 键 词： | 麦麸 小麦矮腥黑穗病菌 孢子提取 |
| 文章编号： | 0412-0914(2002)04-0319-07 |
| 修稿时间： | 2002年2月27日 |
METHOD OF EXTRACTING SPORES OF Tilletia controversa FROM BRAN |
| |
| ZHANG Gui ming,LU Jian,TIAN Zi zhang,CHEN Zhi nan,DENG Qiong,LI Yi nong.METHOD OF EXTRACTING SPORES OF Tilletia controversa FROM BRAN[J].Acta Phytopathologica Sinica,2002,32(4):319-325. |
| |
| Authors: | ZHANG Gui ming LU Jian TIAN Zi zhang CHEN Zhi nan DENG Qiong LI Yi nong |
| |
| Institution: | Shenzhen Entry & Exit Inspection and Quarantine Bureau, Shenzhen, Guangdong 518010, China |
| |
| Abstract: | Methods of extracting spores of Tilletia controversa from bran were studied. The methods "sieving-α-amylase degradation","sieving-density gradient centrifugation" and "sieving-α-amylase degradation-density gradient centrifugation" could be used to extract the spores of Tilletia controversa (TCK) from bran. The number of TCK spores extracted depended on the usage of sieving, α-amylase and density gradient centrifugation while the purity mainly on the density gradient centrifugation. 83.88%-94.09% of bran could be removed by the method of double layer gauze and 5 different combinations of sieving treatments. 95.01%-99.00% of bran could be removed and 19.2%-51.7% of TCK spores could be extracted by the method of "sieving-α-amylase degradation". As for the method of "sieving-density gradient centrifugation", only the treatment of "60 meshes + 200 meshes+300 meshes+30 μm+11 μm" could work so that 7.2% pure spores were obtained after further density gradient centrifugation which didn't influence the reticulum height of spores, autofluorescence and germination of spores. Likewise, as to the method of "sieving-α-amylase degradation-density gradient centrifugation", the treatment of "60 meshes+200 meshes+300 meshes" and "60 meshes+200 meshes+300 meshes+30 μm+11 μm" could work so that 18.8% and 12.2% of pure spores were obtained after further density gradient centrifugation which didn't influence the reticulum height of spores. The most effective condition for α-amylase to degrade insoluble starches is at 71℃ and 350 r/min for 3 mins.These methods have been extensively applied in the routine quarantine at the port. |
| |
| Keywords: | bran Tilletia controversa extraction of spores |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
