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γ射线诱变烟草花药培养的突变体
引用本文:佟道儒,贾兴华.γ射线诱变烟草花药培养的突变体[J].核农学报,1991,5(4):193-198.
作者姓名:佟道儒  贾兴华
作者单位:中国农业科学院烟草研究所,中国农业科学院烟草研究所 青州 262500 ,青州 262500
摘    要:本研究利用~(60)Coγ射线辐照烟草单核靠边期的花药,诱导培养突变体。结果表明,1kR左右的γ射线对花粉的出苗率有促进效应;3kR时,花药出苗时间延长,出苗花药率和花药产苗率明显降低,视为半致死剂量;6kR时,虽有部分花药可以出苗,但花药产苗率极低。诱变处理当代出现白花突变体,其后代的品质比亲本品种有明显改善,但抗病力下降。经试验,该突变系在生产上有实用价值。

关 键 词:γ射线    烟草    花药    白花突变体
收稿时间:2009-12-31
修稿时间:2009-12-31

MUTANT IN TOBACCO ANTHER CULTURE INDUCED BY ~(60)COγr-RAYS
Abstract:The tobacco anthers at uninucleate eccentric stage were irradiated by 60CoY-rays for the purpose of inducing desirable mutants.The results showed that the induction frequency of plantlets increased following 1kR of 60Co γ-rays treatment.Howevr,the time of plantlet induction was delayed and the percentage of responding anthers as well as the number of plantlets induced per anther significantly decreased after 3kR of 60Co γ-ray radiation which was considered as a semilethal exposure.The plantlet numbers induced per anther were extremely low following 6kR of 60Coγ-ray radiation.A white flower mutant appeared in the induced progenies.-The tobacco leaf quality of,this mutant were significa'ntly imporoved as compared with its parental line.The mutant line has been tested and proved to have commercial value though the resistance to the black shank of tobacco slightly decreased as compared with the parental line.
Keywords:60CoY-rays  tobacco  anthers  white flower mutant
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