| ~(15)N天然丰度法测量豆科牧草共生固氮的评估 |
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| 引用本文： | 姚允寅,陈明,马昌燐,王志东,侯景琴,张丽红,骆永云.~(15)N天然丰度法测量豆科牧草共生固氮的评估[J].核农学报,1991,5(3):139-145. |
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| 作者姓名： | 姚允寅 陈明 马昌燐 王志东 侯景琴 张丽红 骆永云 |
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| 作者单位： | 中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所,中国农业科学院原子能利用研究所 北京 100094 ,北京 100094 ,北京 100094 ,北京 100094 ,北京 100094 ,北京 100094 ,北京 100094 |
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| 摘 要： | 无氮营养液砂培7个品种苜蓿茎叶的固氮分馏因数β值为1.0000～1.0015(δ~(15)N为-0.05～-1.47‰),白三叶、绿豆和银合欢的β值分别为0.9979、0.9983和1.0018(δ~(15)N分别为2.15、1.74及-1.81‰)。根据~(45)N天然丰度的变化,估测了田间生长苜蓿的固氮能力,表明6个品种的共生固氮能力不同。格洛里及润布勒苜蓿两品种最高,保定、阿尔贡奎因及明托苜蓿3个品种次之,秘鲁苜蓿最低。苜蓿不同时期的固氮活性有变化,6、7月间固氮活性达高峰。根据所测豆科牧草茎叶的δ~(15)N值,可以对豆科牧草进行定性或半定量水平上的固氮研究,以筛选高效固氮牧草和检测野生固氮资源。本文还对不固氮参照植物和不同方法对％Ndfa估计位的影响进行了讨论。
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| 关 键 词： | ~(15)N天然丰度 共生固氮 豆科牧草 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
EVALUATION OF NATURAL ~(15)N ABUNDANCE METHOD IN ESTIMATING SYMBIOTIC DINITROGEN FIXATION BY LEGUMINOUS GRASSES |
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| Abstract: | Natural 15N abundance method was used to estimate contribution of symbiotic dinitrogen fixation by leguminous grasses.With the method the expensive 15N fertilizer did not need to be applied to the soil and the normal ecosystem was not disturbed.Collecting samples of shoots of leguminous grasses and measuring the content of 15N in.them were all to do for estimating potential of symbiotically fixed N2.Isotopic fractionation associated with N2 fixation by legumes was studied.Values for 7 cultivars of alfalfa were ranged detween 1.0000~1.0015(δ15N values were-0.05~-1.47‰);and the values for white clover,mung bean and whitepopinac leadtree were 0.9979,0.9983 and 1.0018 (δ16N values.2.15,1.74 and-1.81%0) respectively.According to the δ15N values of grasses tested,the potential of N2 fixation for 6 cultivars of alfalfa was estimated.Glory and Rambler had higher potential of N2 fixation;Baoding,Aigonquin and Minto had lower potential,and Peru was the lowest.N2 fixing activity of alfalfa varied with different periods.The peak was found between June and July.Effects of non-N2-fixing references and different methods on estimates of %Ndfa of leguminous grasses were also discussed in the paper. Natural 15N abnudance,symbiotical dinitrogen fixation,leguminous grasses |
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| Keywords: | Natural 15N abundance symbiotic dinitrogen fixation leguminous grasses |
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