| 基于DNA条形码对粘虫四种近缘种的鉴定与亲缘关系分析 |
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| 引用本文： | 陈秀洁,程李莉,江幸福,程云霞,张蕾,罗礼智.基于DNA条形码对粘虫四种近缘种的鉴定与亲缘关系分析[J].植物保护学报,2017,44(3):385-392. |
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| 作者姓名： | 陈秀洁 程李莉 江幸福 程云霞 张蕾 罗礼智 |
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| 作者单位： | 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 |
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| 基金项目： | 国家公益性行业（农业）科研专项（201403031），国家自然科学基金（31672019），北京市自然科学基金（6172030） |
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| 摘 要： | 为开发快速、准确的粘虫Myth imna separata (Walker)近源种鉴定技术,通过线粒体COI基因DNA条形码技术对在野外采集的4种粘虫近缘种——白点粘夜蛾Leucania loreyi、淡脉粘夜蛾L.roseilinea、瘠粘夜蛾 L.pallidior和虚研夜蛾Aletia pseudaletiana的COI基因片段进行序列分析,并对4种粘虫近缘种同粘虫和GenBank中其它6种粘虫近缘种之间的亲缘关系进行了分析.结果表明,克隆得到白点粘夜蛾、淡脉粘夜蛾、瘠粘夜蛾和虚研夜蛾大小为658 bp的COI基因片段,与粘虫COI基因片段的核苷酸序列同源性依次为93.31％、92.86％、91.95％和90.88％,遗传距离分别为0.067、0.071、0.081和0.091;所编码的219个氨基酸序列同源性依次为100.00％、100.00％、99.09％和99.09％.与其它6种粘虫近缘种的COI基因片段序列分析中,白点粘夜蛾和淡脉粘夜蛾与粘虫、一点粘虫Mythimna unipuncta、仿劳粘夜蛾L.insueta、白脉粘夜蛾L.venalba聚为1个亚群,亲缘关系较近;瘠粘夜蛾和虚研夜蛾与白杖研夜蛾A.lalbum、角线研夜蛾A.conigera、秘夜蛾M.turca聚为1个亚群,亲缘关系较近.表明4种近缘种与粘虫的亲缘关系由远到近依次为白点粘夜蛾、淡脉粘夜蛾、粘瘠粘夜蛾和虚研夜蛾.
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| 关 键 词： | 粘虫 近缘种 COI基因 亲缘关系 |
| 收稿时间： | 2016/4/6 0:00:00 |
Identification and phylogenetic analysis of four closely related species of the oriental armyworm Mythimna separata based on DNA barcoding |
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| Chen Xiujie,Cheng Lili,Jiang Xingfu,Cheng Yunxi,Zhang Lei and Luo Lizhi.Identification and phylogenetic analysis of four closely related species of the oriental armyworm Mythimna separata based on DNA barcoding[J].Acta Phytophylacica Sinica,2017,44(3):385-392. |
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| Authors: | Chen Xiujie Cheng Lili Jiang Xingfu Cheng Yunxi Zhang Lei and Luo Lizhi |
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| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China and State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | In order to study the relationship of four field-collected closely related species of the oriental armyworm Mythimna separata and other six closely related species in the GenBank,COI barcode sequences of the four armyworm species were analyzed.The results showed that the COI gene fragments of the four species of Noctuidae were all 658 bp in length.The COI sequences of Leucania loreyi,L.roseilinea,L.pallidior and Aletia pseudaletiana shared 93.31%,92.86%,91.95% and 90.88% identity with that of M.separata,respectively,and 100.00%,100.00%,99.09% and 99.09% in amino acid sequences.The pairwise distances between the COI gene sequences of the four Noctuidae species and M.separata were 0.067,0.071,0.081 and 0.091,respectively.In addition,the COI gene fragments of the four closely related species and other six armyworm sibling species were analyzed,and the results showed that L.loreyi and L.roseilinea had a closer phylogenetic relationship with M.separata,M.unipuncta,L.insueta,and L.venalba;L.pallidior and A.pseudaletiana had a closer relationship with A.lal-bum,A.conigera and M.turca.Taking the genetic distance analysis,homologous identity analysis and phylogenetic tree analysis together,the four species L.loreyi,L.roseilinea,L.pallidior and A.pseudaletiana had closer genetic relationship with M.separata,in order. |
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| Keywords: | Mythimna separata closely related species COI gene genetic relationship |
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