| 二化螟Cry1Ac汰选品系对不同Bt蛋白的敏感性 |
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| 引用本文： | 何珊,徐杨洋,韩兰芝,贾变桃,彭于发.二化螟Cry1Ac汰选品系对不同Bt蛋白的敏感性[J].植物保护学报,2017,44(3):371-376. |
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| 作者姓名： | 何珊 徐杨洋 韩兰芝 贾变桃 彭于发 |
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| 作者单位： | 1. 山西农业大学农学院,太谷030801;中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193;2. 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193;3. 山西农业大学农学院,太谷,030801 |
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| 基金项目： | 国家转基因生物新品种培育重大专项（2016ZX08011-001，2014ZX0801104B），国家自然科学基金（31572336） |
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| 摘 要： | 为明确靶标害虫对Bt蛋白的抗性及对不同类型Bt蛋白的交互抗性,采用生物测定法进行了二化螟Chilo suppressalis(Walker)敏感品系和Cry1Ac汰选品系在Cry1Ac毒饲料上的时间-死亡率反应、对不同Bt杀虫蛋白的敏感性及不同Bt蛋白复配组合对二化螟毒力效果的研究。结果表明,二化螟Cry1Ac汰选品系已对36.67μg/mL(LC_(50))的Cry1Ac杀虫蛋白产生了一定的适应性,但对308.69μg/mL(LC90)的Cry1Ac蛋白反应仍较敏感。Cry1Ac敏感品系和汰选品系对Cry1Ab蛋白的LC_(50)分别为1.40μg/mL和3.86μg/mL,二者差异显著,但对Cry1Ca(1.63μg/mL和1.73μg/mL)或Cry2Aa(127.48μg/mL和144.50μg/mL)的LC_(50)差异不显著,即Cry1Ac汰选品系与Cry1Ab存在明显的交互抗性,但与Cry1Ca和Cry2Aa不存在交互抗性。在不同Bt蛋白复配组合中(1∶1复配),Cry1Ab+Cry1Ca、Cry1Ab+Cry2Aa和Cry1Ca+Cry2Aa增效作用最为显著。表明抗虫基因cry1Ca和cry2Aa可作为双价转基因抗虫水稻研发的候选基因。
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| 关 键 词： | 二化螟 Cry1Ac汰选品系 Bt杀虫蛋白 敏感性变化 |
| 收稿时间： | 2015/11/6 0:00:00 |
Variation in the susceptibility of a Chilo suppressalis Cry1Ac-selective strain to different Bacillus thuringiensis insecticidal proteins |
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| He Shan,Xu Yangyang,Han Lanzhi,Jia Biantao and Peng Yufa.Variation in the susceptibility of a Chilo suppressalis Cry1Ac-selective strain to different Bacillus thuringiensis insecticidal proteins[J].Acta Phytophylacica Sinica,2017,44(3):371-376. |
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| Authors: | He Shan Xu Yangyang Han Lanzhi Jia Biantao and Peng Yufa |
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| Institution: | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China,College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China and State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | The time-mortality responses of Cry 1Ac-susceptible and-selective Chilo suppressalis strains,variations in the two strains' susceptibility to different Bacillus thuringiensis (Bt) insecticidal proteins,and synergism of different Bt protein combinations on C.suppressalis were studied.In the time-mortality response experiment,the Cry1Ac-selective strain had a degree of tolerance to the LC50 treatment with 36.67 μg/mL and absolute susceptibility to the LC90 treatment with 308.69 μg/mL of the Cry1Ac-containing diet.The LC50 values of Cry1Ac-susceptible and-selective strains to Cry 1Ab were 1.40 μg/mL and 3.86 μg/mL,respectively,showing significant differences between the two values.However,there was no significant difference in the LC50 values of Cry1Ca (1.63,1.73 μg/mL) or Cry2Aa (127.48,144.50 μg/mL) between Cry1Ac-susceptible and-selective strains.The Cry1Ac-selective strain of C suppressalis exhibited cross-resistance to Cry1Ab,but not to Cry1Ca or Cry2Aa.The 1 ∶ 1 combinations of Cry1Ab+Cry1Ca,Cry1Ab+Cry2Aa,and Cry1Ca+Cry2Aa showed significant synergistic interactions against C.suppressalis,indicating that insect-resistant gene,cry1Ca or cry2Aa may be used to develop pyramided genetically modified rice. |
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| Keywords: | Chilo suppressalis Cry1Ac-selective strain Bt insecticidal protein variation in susceptibility |
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