| 河南省部分地区麦田荠菜对苯磺隆的抗性水平及抗性靶标分子机制 |
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| 引用本文： | 高新菊,王恒亮,马毅辉,陈威,秦光宇,宋语娇.河南省部分地区麦田荠菜对苯磺隆的抗性水平及抗性靶标分子机制[J].植物保护学报,2017,44(3):501-508. |
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| 作者姓名： | 高新菊 王恒亮 马毅辉 陈威 秦光宇 宋语娇 |
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| 作者单位： | 1. 河南省农业科学院植物保护研究所,河南省农作物病虫害防治重点实验室,农业部华北南部作物有害生物综合治理重点实验室,河南省作物保护国际联合实验室,河南省生物农药工程研究中心,郑州450002;2. 河南农业职业学院,郑州,451450 |
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| 基金项目： | 河南省农业科学院自主创新专项基金（2013ZZ22，2016ZC43），河南省博士后基金科研资助项目（2013046） |
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| 摘 要： | 为明确河南省部分地区麦田荠菜Capsella bursa-pastoris对苯磺隆的抗性水平及抗性靶标分子机制,采用整株生物法测定了12个荠菜种群的抗性水平,并对乙酰乳酸合成酶(acetolactate synthase,ALS)离体活性和ALS基因突变进行了测定分析。结果表明,商丘市民权县花园村(MQ)、周口市西华县小于楼村(XH)、平顶山市叶县穆寨村(YX)、许昌市长葛市董庄村(CG)采集的荠菜种群对苯磺隆产生了较高的抗性,GR_(50)分别为129.14、110.67、62.91和85.29 g/hm~2,抗性倍数分别为215.23、184.45、104.85和142.15倍;ALS离体活性测定所得I_(50)分别为5.85、4.87、1.38和3.83μmol/L,抗性倍数分别为83.57、69.57、19.71和54.71倍;其余8个种群的GR_(50)在0.60~2.86 g/hm~2之间,抗性倍数在1.00~4.77之间;I_(50)在0.07~0.37μmol/L之间,抗性倍数在1.00~5.29之间。荠菜种群MQ、XH的ALS基因Domain A区域第197位脯氨酸(CCT)均突变为丝氨酸(TCT),荠菜种群CG的第197位脯氨酸(CCT)突变为亮氨酸(CTT),表明靶标ALS基因突变是荠菜对苯磺隆产生抗性的重要原因之一,但荠菜种群YX的ALS基因保守区内暂未发现突变位点,其抗药性可能由其它原因造成。
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| 关 键 词： | 河南省 荠菜 苯磺隆 抗药性 |
| 收稿时间： | 2016/6/2 0:00:00 |
Resistance of Capsella bursa-pastoris to tribenuron-methyl in winter wheat fields in some areas of Henan Province and its molecular mechanism |
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| Gao Xinju,Wang Hengliang,Ma Yihui,Chen Wei,Qin Guangyu and Song Yujiao.Resistance of Capsella bursa-pastoris to tribenuron-methyl in winter wheat fields in some areas of Henan Province and its molecular mechanism[J].Acta Phytophylacica Sinica,2017,44(3):501-508. |
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| Authors: | Gao Xinju Wang Hengliang Ma Yihui Chen Wei Qin Guangyu and Song Yujiao |
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| Institution: | Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides Engineering Research Center of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan Province, China,Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides Engineering Research Center of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan Province, China,Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides Engineering Research Center of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan Province, China,Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides Engineering Research Center of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan Province, China,Henan Key Laboratory of Crop Pest Control, IPM Key Laboratory in Southern Part of North China for Ministry of Agriculture, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides Engineering Research Center of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan Province, China and Henan Vocational College of Agriculture, Zhengzhou 451450, Henan Province, China |
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| Abstract: | To investigate the resistance levels of Capsella bursa-pastoris to tribenuron-methyl in winter wheat fields in some areas of Henan Province and its molecular mechanism,12 C.bursa-pastoris populations were assessed with three methods:whole-plant bioassay,acetolactate synthase (ALS) activity in vitro assay and analysis of ALS mutation.The results showed that C.bursa-pastoris populations from Huayuan Village,Minquan County in Shangqiu City,Xiaoyulou Village,Xihua County in Zhoukou City,Muzhai Village,Ye County in Pingdingshan City and Dongzhuang Village,Changge City in Xuchang City had higher level of resistance than other populations.The GR50 values determined with the method of whole-plant bioassay were 129.14,110.67,62.91 and 85.29 g/hm2,respectively,and the corresponding relative resistance indexes were 215.23,184.45,104.85 and 142.15,respectively.The I50 values determined with in vitro assay of ALS activity were 5.85,4.87,1.38 and 3.83 μmol/L,respectively,and the corresponding relative resistance indexes were 83.57,69.57,19.71 and 54.71,respectively.The GR50 of other eight populations ranged from 0.60 g/hm2 to 2.86 g/hm2,and the corresponding resistance indexes ranged from 1.00 to 4.77;the I50 values were in the range of 0.07-0.37 μmol/L and the cal-culated resistance indexes in the range of 1.00-5.29.ALS sequence analysis indicated that proline (CCT) at position 197 in Domain A of the ALS gene was substituted by serine (TCT) in the populations MQ and XH,and leucine (CTT) in the population CG.The Pro197 mutation inALS could be one of important reasons for the observed high resistance to tribenuron-methyl.In contrast,no point mutations were indentified in the ALS gene from the population YX,indicating other mechanisms might be responsible for its resistance. |
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| Keywords: | Henan Province Capsella bursa-pastoris tribenuron-methyl herbicide resistance |
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