| 猕猴桃种质资源RAPD分析 |
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| 引用本文： | 贾兵,朱立武,余兴,陆丽娟,王艳芳.猕猴桃种质资源RAPD分析[J].安徽农业大学学报,2005,32(3):381-384. |
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| 作者姓名： | 贾兵 朱立武 余兴 陆丽娟 王艳芳 |
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| 作者单位： | 安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036;安徽农业大学果树学重点实验室,合肥,230036 |
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| 摘 要： | 以猕猴桃10个种42个样品叶片为试验材料,采用改良CTAB法提取基因组DNA,优化确立RAPD-PCR的反应体系.从80个随机引物中筛选出38个引物进行扩增,应用SPSS 11.0 for Windows 软件进行聚类分析.结果表明,美味猕猴桃和中华猕猴桃亲缘关系近,可将其归为同一个种;品种之间确有按原产地聚类的趋势;"海沃德"与其营养系品种"皖翠"有14个引物扩增出了18条特异性条带,变异条带较多.
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| 关 键 词： | 猕猴桃 品种 资源 RAPD |
| 文章编号： | 1672-352X(2005)03-0381-04 |
| 修稿时间： | 2004年10月19日 |
RAPD Analysis on Germplasm Resources in Genera Actinidia |
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| Jia Bing,ZHU Li-wu,Yu Xing,LU Li-juan,WANG Yan-fang.RAPD Analysis on Germplasm Resources in Genera Actinidia[J].Journal of Anhui Agricultural University,2005,32(3):381-384. |
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| Authors: | Jia Bing ZHU Li-wu Yu Xing LU Li-juan WANG Yan-fang |
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| Abstract: | The genomic DNA of 10 Actinidia species including 42 samples were extracted by the improved CTAB method. The parameters in RAPD-PCR were optimized. Thirty-eight arbitrary primers used in the study were screened from 80 ones. According to the data of RAPD-PCR,the cluster analysis was done by the SPSS (11.0) for Windows. The results showed that the relationship between A.chinensis and A.deliciosa was so close that they should be considered as the same species, and the classification between cultivars was accordant with their geographical distribution, and 18 characteristic bands between "Hayward"and "Wancui"were amplified in 14 primers by RAPD-PCR. |
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| Keywords: | Actinidia cultivars germplasm RAPD |
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