| H5N1亚型禽流感病毒低剂量感染小鼠发病模型的建立 |
| |
| 引用本文： | 李旭勇,郭晶,施建忠,李雁冰,陈化兰.H5N1亚型禽流感病毒低剂量感染小鼠发病模型的建立[J].中国预防兽医学报,2011,33(7). |
| |
| 作者姓名： | 李旭勇 郭晶 施建忠 李雁冰 陈化兰 |
| |
| 作者单位： | 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
| |
| 摘 要： | 为模拟哺乳动物感染H5亚型高致病性禽流感病毒(HPAIV)的发病进程,本研究采用对哺乳动物高度致病的H5N1亚型HPAIV株A/bar-headed goose/Qinghai/3/05 (BHG/3/05),以低剂量鼻腔接种小鼠,观察发病、存活、病毒复制及组织病理损伤情况.结果显示,100.4 EID50即能够100％感染小鼠,但发病表现缓慢,死亡延迟至8d以后,存活达60％;体内病毒复制可持续10 d以上,感染后前3d病毒的增殖限于呼吸道,随后扩散至脑、脾、肾等其他器官;组织病理学观察肺脏早期表现出渗出性炎症,第10d发展为典型的间质性肺炎.本研究结果为探讨人禽流感的病理发生机制提供了具有价值的模型.
|
| 关 键 词： | H5N1高致病性禽流感病毒 小鼠 感染模型 |
Pathogenicity of a low-dosage inoculation of H5N1 avian influenza virus in mice |
| |
| LI Xu-yong,GUO Jing,SHI Jian-zhong,LI Yan-bing,CHEN Hua-lan.Pathogenicity of a low-dosage inoculation of H5N1 avian influenza virus in mice[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(7). |
| |
| Authors: | LI Xu-yong GUO Jing SHI Jian-zhong LI Yan-bing CHEN Hua-lan |
| |
| Institution: | LI Xu-yong,GUO Jing,SHI Jian-zhong,LI Yan-bing,CHEN Hua-lan (State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
| |
| Abstract: | To imitate the natural infection of H5N1 subtype avian influenza virus (AIV) in animals, BALB/c mice were inoculated with a low-dosage (100.4 EID50) of H5N1 AIV A/bar-headed goose/3/05 (BHG/3/05) strain and were observed for weight gain and mortality. Different organs of the mice were also collected at different time points after infection for virus titration and pathologic examination. The results indicated that all of the inoculated mice were successfully infected by the virus, and 60% of mice survived du... |
| |
| Keywords: | highly pathogenic H5N1 influenza virus mice infection model |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
