| 甲型H1N1流感病毒株的分离鉴定以及PR8株的相关基因对重组病毒增殖能力的影响 |
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| 引用本文： | 李建辉,刘春国,张云,刘明.甲型H1N1流感病毒株的分离鉴定以及PR8株的相关基因对重组病毒增殖能力的影响[J].中国预防兽医学报,2011,33(7). |
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| 作者姓名： | 李建辉 刘春国 张云 刘明 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 黑龙江省自然科学基金，现代农业水禽技术体系 |
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| 摘 要： | 为分离流感病毒,本研究将疑似甲型流感患者的咽喉拭子接种SPF鸡胚尿囊腔,经检测收获的鸡胚尿囊液具有血凝活性.采用流感病毒通用引物进行PCR扩增出8个基因节段,经测序与GenBank中登录的序列比对后确定该病毒株为2009年爆发的甲型H1N1流感病毒,并命名为A/Harbin/LM/2009 (H1N1),简称LM株.由于LM分离株在鸡胚中增殖能力比较弱,为提高其在鸡胚中的增殖能力,我们利用反向遗传技术将LM株的HA和NA基因与高增殖特性的人流感病毒细胞高产株PR8的另外6个节段进行“6+2”重配,获得拯救病毒,但该重配病毒血凝价并未显著提高.为进一步鉴定影响病毒增殖能力的基因,本研究将LM株所有节段以“7+1”的方式逐一与PR8的7个片段搭配构成完整的流感病毒基因组,分别得到8个重配病毒.结果显示分离株的PB1、PA、HA基因显著降低了重配病毒在鸡胚细胞中的增殖能力.
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| 关 键 词： | 甲型H1N1流感病毒 反向遗传 重配 增殖能力 |
Isolation of an H1N1 influenza A virus and identification of genes influencing on the replication of reassortant virus with PR8 backbone |
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| LI Jian-hui,LIU Chun-guo,ZHANG Yun,LIU Ming.Isolation of an H1N1 influenza A virus and identification of genes influencing on the replication of reassortant virus with PR8 backbone[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(7). |
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| Authors: | LI Jian-hui LIU Chun-guo ZHANG Yun LIU Ming |
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| Institution: | LI Jian-hui,LIU Chun-guo,ZHANG Yun,LIU Ming(State Key Laboratory of Veterinary Biotechnology,Animal Influenza Laboratory of the Ministry of Agricultural,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | In this study, a virus was isolated from an adult throat swab in Harbin and identified as an H1N1 influenza virus by hemagglutination test and gene sequencing, designated A/Harbin/LM/2009 (H1N1). To improve the replication capacity of the isolate in embryoed eggs, we utilized reverse genetics technique to construct 6+2 reassortant virus, which included HA and NA segments of the isolate and the remaining segments of high-yield influenza virus PR8, as a result the HA titer of the reassortment virus was not si... |
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| Keywords: | H1N1 influenza A virus reverse genetics reassortment replication capacity |
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