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| 雏鸭源多杀性巴氏杆菌的分离鉴定和荚膜PCR分型 | |
| 引用本文： | 孙翠平,王金良,李书光,赵蕾,贾娟,莫玲,沈志强.雏鸭源多杀性巴氏杆菌的分离鉴定和荚膜PCR分型[J].中国预防兽医学报,2011,33(7). |
| 作者姓名： | 孙翠平 王金良 李书光 赵蕾 贾娟 莫玲 沈志强 |
| 作者单位： | 1. 山东绿都生物科技有限公司,山东滨州,256600 2. 山东省滨州畜牧兽医研究院,山东滨州,256600 3. 山东绿都生物科技有限公司,山东滨州 256600;山东省滨州畜牧兽医研究院,山东滨州 256600 |
| 摘 要： | 本研究从河南某肉鸭养殖场感染鸭肝炎病毒的10日龄病死鸭肝脏中分离到一株致病菌,经菌落形态学观察、培养特性、生化反应、致病性回归试验和SPF鸡攻毒试验,结合多杀性巴氏杆菌(Pasteurella multocida,Pm) kmt1种特异性基因检测方法进行鉴定,并通过16S rRNA序列测定进行同源性分析.结果显示该分离株为Pm,命名为Pm-Y.致病性回归试验表明低浓度的Pm-Y只引起部分10日龄雏鸭发病死亡.SPF鸡攻毒试验显示,Pm-Y与国内标准强毒株C48-1的致病力相近,16S rRNA序列系统发育树分析表明Pm-Y与Pm多杀亚种和杀禽亚种亲缘关系最近.参考荚膜血清特异性基因hyaD-hyaC、bcbD、dcbF、ecbJ和fcbD合成引物,扩增荚膜血清型特异性基因,对Pm-Y进行荚膜分型鉴定为A型Pm.本研究是国内首例从感染鸭肝炎病毒10日龄雏鸭肝脏中分离到Pm的报道. |
| 关 键 词： | 多杀性巴氏杆菌 10日龄肉鸭 荚膜A型 分离鉴定 |
Isolation and identification of a Pasteurella mutocida from ducklings |
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| SUN Cui-ping,WANG Jin-liang,LI Shu-guang,ZHAO Lei,JIA Juan,MO Ling,SHEN Zhi-qiang.Isolation and identification of a Pasteurella mutocida from ducklings[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(7). | |
| Authors: | SUN Cui-ping WANG Jin-liang LI Shu-guang ZHAO Lei JIA Juan MO Ling SHEN Zhi-qiang |
| Institution: | SUN Cui-ping1,WANG Jin-liang2,LI Shu-guang2,ZHAO Lei2,JIA Juan1,MO Ling2,SHEN Zhi-qiang1,2 (1. Shandong LvDu Bio-sciences Technology Co.,Ltd,Binzhou 256600,China,2. Shandong Binzhou Animal Sciences & Veterinary Medicine Academy,China) |
| Abstract: | A Pasteurella multocida was isolated from the liver of 10-day-old meat duckling infected with hepatitisy virus from a duck farm in Henan province. The isolate, designated Pm-Y, was and identified by colonial morphology, culture characteristic, biochemistry reaction, pathogenicity test on 10-day-old meat ducklings and SPF chicken. Subsequently, species-specific of the Pm-Y and the 16S rRNA sequence was amplified and sequenced. The results indicated that Pm-Y was lethal to ducklings and similar to standard ve... |
| Keywords: | Pasteurella mutocida 10-day-old meat duck capsular serotype A isolation and identification |
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