| 鸡胚胎性病原茵多重PCR检测方法的建立 |
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| 引用本文： | 谭燕玲,朱瑞良,王慧,王新建,魏凯,孙振红,盛鹏程.鸡胚胎性病原茵多重PCR检测方法的建立[J].中国预防兽医学报,2011,33(5). |
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| 作者姓名： | 谭燕玲 朱瑞良 王慧 王新建 魏凯 孙振红 盛鹏程 |
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| 作者单位： | 山东农业大学动物科技学院山东省动物生物技术与疫病防治重点实验室,山东,泰安,271018 |
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| 基金项目： | 国家自然科学基金，山东省自然科学基金项目 |
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| 摘 要： | 为建立同时检测禽波氏杆菌(Bordetella avium)、沙门氏茵(Salmonella)、大肠杆菌(Escherichia coli)和绿脓杆菌(Pseudomonas aeruginosa)4种导致鸡胚死亡病原菌的多重PCR方法,本研究根据B.aviun的ompA基因、Salmonella的invA基因、E.coli的phoA基因和P.aeruginosa的toxR基因序列,各设计一对特异性引物进行多重PCR反应,并对反应体系和条件进行优化.结果显示,4对引物分别扩增出597bp、724bp、372bp和278bp的目的条带;并且特异性强不与其他非目的茵发生反应.经优化B.aviun、P.aeruginosa和E.coli多重PCR检测灵敏度达到104cfu/mL,而Salmonella为103cfu/mL.本研究建立的多重PCR方法为相关病原茵的快速检测提供方法.
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| 关 键 词： | 禽波氏杆菌 沙门氏茵 大肠杆菌 绿脓杆菌 多重PCR |
Establishment of multiplex PCR detection for pathogens of chicken embryos |
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| TAN Yan-ling,ZHU Rui-liang,WANG Hui,WANG Xin-jian,WEI Kai,SUN Zhen-hong,SHENG Peng-cheng.Establishment of multiplex PCR detection for pathogens of chicken embryos[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(5). |
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| Authors: | TAN Yan-ling ZHU Rui-liang WANG Hui WANG Xin-jian WEI Kai SUN Zhen-hong SHENG Peng-cheng |
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| Institution: | TAN Yan-ling,ZHU Rui-liang,WANG Hui,WANG Xin-jian,WEI Kai,SUN Zhen-hong,SHENG Peng-cheng (Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China) |
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| Abstract: | To establish a multiplex PCR assay for simultaneous detecation of Bordetella avium, Escherichia coli, Salmonella and Pseudomonas aeruginosa in infected embryos, four pairs of specific primers were designed and synthesized based on the genes of ompA in B. avium, invA in Salmonella, phoA in E. coli and toxR in P. aeruginosa for detection of these pathogenic bacteria by multiplex PCR. Test results indicated that the PCR products were 597 bp for B. avium, 724 bp for Salmonella, 372 bp for E. coli, and 278 bp fo... |
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| Keywords: | Bordetella avium Salmonella Escherichia coli Pseudomonas aeruginosa multiplex PCR |
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