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寡聚糖诱导黄瓜对白粉病抗病反应的超微结构研究
引用本文:马青,孙辉,杜昱光,赵小明,商鸿生.寡聚糖诱导黄瓜对白粉病抗病反应的超微结构研究[J].植物病理学报,2004,34(6):525-530.
作者姓名:马青  孙辉  杜昱光  赵小明  商鸿生
作者单位:1 西北农林科技大学植物保护学院, 陕西杨凌 712100;2 中国科学院大连化学物理研究所, 辽宁大连 116023
基金项目:国家高技术研究发展计划(863计划),中国科学院知识创新工程项目
摘    要: 中科6号(2%氨基寡糖素)处理黄瓜植株叶片,5 d后接种白粉菌Sphaerotheca fuliginea(Schlecht.) Poll.,可诱导黄瓜Cucumis sativus产生对白粉病的抗病性,寄主细胞对病原菌的侵入产生了防卫反应结构和物质以及过敏性坏死反应。表现为寄主细胞壁加厚,染色加深,寄主细胞壁下产生多层次结构的乳突,在寄主细胞壁与质膜之间有黑色物质沉积;吸器外质膜皱褶,染色加深,吸器外基质中出现染色加深的颗粒状电子沉积物;寄主细胞质紊乱,细胞器解体,整个寄主细胞解体、坏死。

关 键 词:黄瓜  白粉病菌  寡聚糖  诱导抗病性  超微结构  
文章编号:0412-0914(2004)06-0525-06
收稿时间:2003-10-29
修稿时间:2003年10月29

Induction of oligosaccharide to ultrastructure of cucumber resistance to powdery mildew fungus
MA Qing,SUN Hui,DU Yu-guang,ZHAO Xiao-ming,SHANG Hong-sheng.Induction of oligosaccharide to ultrastructure of cucumber resistance to powdery mildew fungus[J].Acta Phytopathologica Sinica,2004,34(6):525-530.
Authors:MA Qing  SUN Hui  DU Yu-guang  ZHAO Xiao-ming  SHANG Hong-sheng
Institution:1 College ofPlant Protection, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China;2 Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning 116023, China
Abstract:Five days after being treated with amino oligosaccharin, the cucumber leaves were inoculated with cucumber powdery mildew, Sphaerotheca fuliginea (Schlecht. ) Poll. , to induce the cucumber resistance to powdery mildew. The host cells produced defense structures and material related to infection and hypersensitive response. The host cell wall was thickened and deeply stained, several layers of papilla structure produced under the cell wall, dark material deposited between the cell wall and plasmalemma; extrahaustorial plasmalemma deeply stained and extrahaustorial matrix appositions deposited with large amount of electron-dense material; cytoplasm disordered, host organelles disintegrated and finally the whole host cell disintegrated and necrotized.
Keywords:cucumber  Sphaerotheca fuliginea  oligosaccharide  induced resistance  ultrastructure
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