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| 盐度对俄罗斯鲟幼鱼血清渗透压、离子含量及鳃丝 Na+/K+-ATP 酶活力的影响 | |
| 引用本文： | 屈亮,庄平,章龙珍,赵峰,刘鉴毅,王妤.盐度对俄罗斯鲟幼鱼血清渗透压、离子含量及鳃丝 Na+/K+-ATP 酶活力的影响[J].中国水产科学,2010,17(2):243-251. |
| 作者姓名： | 屈亮 庄平 章龙珍 赵峰 刘鉴毅 王妤 |
| 作者单位： | 1. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090;上海海洋大学水产与生命学院,上海,201306 2. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090;上海海洋大学水产与生命学院,上海,201306;大连水产学院生命科学与技术学院,辽宁大连,116023 3. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090 4. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业资源与生态重点开放实验室,上海,200090;大连水产学院生命科学与技术学院,辽宁大连,116023 |
| 基金项目： | 浙江省科技计划项目，农业科技成果转化资金资助项目，中央级公益性科研院所基本科研业务费专项资金资助项目 |
| 摘 要： | 研究了急性盐度胁迫对7月龄俄罗斯鲟(Acipenser gueldenstaedtii)幼鱼体质量(86.1±18)g]鳃丝Na+/K+-ATP酶活力、血清渗透压和血清离子(Na+、K+、Cl-)的影响。结果表明:幼鱼从淡水直接转入盐度15、20、25水中,96h死亡率分别为72.22%、100%、100%,其他组(盐度0、5、10)无死亡。各盐度组96h血清渗透压、Na+、Cl-浓度随盐度升高而增加,且各盐度组显著高于对照组(P0.05),盐度15组最高,盐度5、10组间各指标不存在显著差异(P0.05)。盐度组血清K+离子在48h中较对照组均显著降低(P0.05),96h时有所回升但仍低于对照组,盐度15与盐度5、10相比血清K+离子有显著性差异(P0.05),盐度5、10之间无显著性差异(P0.05)。盐度组鳃丝Na+/K+-ATP酶活力呈先下降后上升变化,48h为最低,与对照组有显著性差异(P0.05),96h时盐度5、10组鳃丝Na+/K+-ATP酶活力与对照组及组间无显著差异(P0.05),盐度15与对照组及盐度5、10均有显著差异(P0.05)。96h幼鱼的等渗点为303.2mOsm·kg-1,相当于盐度10.06,而Na+及Cl-等离子点分别为146.1mmol·L-1和136.8mmol·L-1,分别相当于盐度9.02和8.95。与盐度15组相比,盐度10及其以下处理组各项检测指标变化幅度相对较小,在幼鱼渗透调节范围之内,盐度10中养殖15d后各项指标与淡水组差异较小,因此7月龄俄罗斯鲟幼鱼已具备在盐度10及以下的咸水中生活的能力,但不能耐受高于10的盐度。 |
| 关 键 词： | 俄罗斯鲟 盐度胁迫 鳃丝Na~+/K~+-ATP酶 血清离子及渗透压 渗透压调节 |
| 修稿时间： | 2010/3/30 0:00:00 |
Influence of salinity change on serum osmolality,ions and gill Na~+/K~+-ATPase activity in juvenile Russian sturgeon(Acipenser gueldenstaedtii) |
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| QU Liang,ZHUANG Ping,ZHANG Longzhen,ZHAO Feng,LIU Jianyi,WANG Yu.Influence of salinity change on serum osmolality,ions and gill Na~+/K~+-ATPase activity in juvenile Russian sturgeon(Acipenser gueldenstaedtii)[J].Journal of Fishery Sciences of China,2010,17(2):243-251. | |
| Authors: | QU Liang ZHUANG Ping ZHANG Longzhen ZHAO Feng LIU Jianyi WANG Yu |
| Institution: | QU Liang 1,2,ZHUANG Ping 1,ZHANG Longzhen 1,ZHAO Feng 1,LIU Jianyi 1,WANG Yu 1,3 (1.Key , Open Laboratory of Marine , Estuarine Fisheries Resource , Ecology,Ministry of Agriculture,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China,2.College of Fisheries , Life Science,Shanghai Ocean University,Shanghai 201316,3.College of Aqua-life Science , Technology,Dalian Fisheries University,Dalian 116023,China) |
| Abstract: | Effects of abrupt salinity changes on gill Na~+-K~+-ATPase activities, survival rates and serum ions of 7-month-old juvenile Russia sturgeon (86.1 g±18 g) were measured at 24 h, 48 h and 96 h after transfer to freshwater (control)and salinity levels of 5,10, 15,20,25. Fish were also acclimated in brackish water (salinity 10)for 2 weeks. The 96 h death rates in salinities 15,20 and 25 were 72.22%, 100% and 100% respectively, and no mortality occurred in lower salinity groups and control group. The changes on serum osmolality, Na~+and Cl~- in salinity groups (salinity 5,10, 15) within 96 hours showed a similar trend. All these indicators elevated with the rising salinities and were significantly higher than those in control (P<0.05). The indicators in salinity 15 were the highest and there were no significant difference between salinity 5 and 10 groups (P>0.05). The serum K~+ concentration in salinity groups decreased at first in 48 hours and then slightly increased between 48 h and 96 h, but they were all significantly lower than the control group within 96 h period (P<0.05), and there were no significant difference between salinities 10 and 5 (P>0.05). Gill Na~+-K~+-ATPase activities in salinity groups also showed a firstly decreasing and then increasing trend during the experimental period, and they were all the lowest at 48 h and significantly lower than those in control (P<0.05). On the 96 h the gill Na~+-K~+-ATPase activity in salinities 5 and 10 showed no significant difference from the control group and each other (P>0.05), but the gill Na~+-K~+-ATPase activity in salinity 15 was significantly different from the lower salinity groups and control group (P<0.05). The isosmotic point was estimated as 303.2 mOsm·kg~(-1) and corresponded to salinity 10.02. The 96 h isoionic points for serum Na~+ and Cl~- were estimated as 146.1 and 136.8 mmol·L~(-1), which corresponded to the salinities 9.02 and 8.95 respectively. As compared to salinity 15 group, salinities 5 and 10 groups showed a minor deviation from the control group in all measurement indicators, which in salinity 10 after 15 d exposure also showed a minor change from the control group, and from these variations in juvenile Russia sturgeon, we can draw a conclusion that 7-month-old Russia sturgeon possess regulatory mechanisms capable of maintaining considerable control of serum ion concentrations in salinity below 10 but unable to survive salinity exceeding 10. |
| Keywords: | Acipenser gueldenstaedtii salinity stress gill Na~+-K~+-ATPase activity serum ions and osmolality osmoregulation |
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