| 马铃薯茎尖脱毒效果影响因素的研究 |
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| 引用本文： | 李凤云.马铃薯茎尖脱毒效果影响因素的研究[J].中国马铃薯,2008,22(4). |
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| 作者姓名： | 李凤云 |
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| 作者单位： | 黑龙江省农业科学研究院马铃薯研究所,黑龙江,克山,161606 |
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| 基金项目： | 黑龙江省农业科学院科研计划项目 |
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| 摘 要： | 以克新2号、克新16号和克新17号等8个马铃薯品种为试验材料,对马铃薯茎尖脱毒效果的影响因素进行了研究。结果表明:不同种类的植物生长调节剂对茎尖的生长有不同的影响,茎尖在⑤号培养基(MS+1.0mg·L-1KT+0.5mg·L-1IAA+0.5mg·L-1GA3)上可不产生愈伤组织,直接成苗,是最适宜的培养基;6～8℃低温预处理和37℃热空气处理对茎尖脱毒有利;茎尖取材来源对成苗率没有影响,对脱毒效果影响较大,但脱毒效果依品种而异;为避免造成损失,需在15个月内准备出用于更换的基础苗。
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| 关 键 词： | 马铃薯 茎尖培养 影响因素 |
Factors Influencing Potato Meristem Culture for Elimination of Viruses |
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| Li Fengyun.Factors Influencing Potato Meristem Culture for Elimination of Viruses[J].Chinese Potato,2008,22(4). |
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| Authors: | Li Fengyun |
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| Abstract: | The eight potato varieties, such as Kexin 2, Kexin 16, and Kexin 17, were used to study the factors influencing potato meristem culture of potato for elimination of viruses. Growth hormone had an important role to play in shoot formation. Meristem cultured on the medium MS+1.0 mg·L-1 KT+0.5 mg·L-1 IAA+0.5 mg·L-1 GA3 produced plantlets directly without callus formation, and therefore it was considered the best medium for shoot induction. Pre-treatment at 6-8℃ and then 37℃ helped eliminate viruses. The source of meristem had no influence on survival rate, but it had some influence on the percentage of plantlets free of viruses. The efficacy of virus elimination was depending on the variety used. Plantlets free of virus had to be replaced by new ones in 15 months. |
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| Keywords: | potato meristem culture factors |
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