| 水稻黄绿叶突变体ygl209的遗传分析与目标基因精细定位 |
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| 引用本文： | 李广贤,姚方印,侯恒军,孙召文,姜明松,朱文银,周学标.水稻黄绿叶突变体ygl209的遗传分析与目标基因精细定位[J].作物学报,2015,41(10):1603-1611. |
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| 作者姓名： | 李广贤 姚方印 侯恒军 孙召文 姜明松 朱文银 周学标 |
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| 作者单位： | 1山东省水稻研究所, 山东济南 250100; 2山东省农业科学院高新技术研究中心, 山东济南 250100; 3济宁市任城区农业局, 山东济宁 272000 |
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| 基金项目： | 本研究由山东省现代农业产业技术体系水稻产业创新团队建设项目(SDAIT-01-016-01), 山东省农业良种工程项目(2013-2015)和山东省农业科学院科技创新重点项目(2014CXZ11)资助。 |
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| 摘 要： | 水稻叶色突变体是研究高等植物光合作用、叶绿体发育和叶绿素代谢的重要材料。从水稻转基因育种材料中国91与镇稻88的BC4F3后代中分离到稳定遗传的粳型黄绿叶突变体ygl209,与野生型亲本镇稻88相比,突变体ygl209在苗期、分蘖期及抽穗期叶片中叶绿素a、叶绿素b和类胡萝卜素含量均显著降低,其中叶绿素b降幅最大;其他农艺性状中抽穗期、株高、有效穗数、主茎穗总粒数、结实率和千粒重无显著变化。遗传分析表明,ygl209的黄绿叶突变性状由1对核隐性基因控制。应用(ygl209/9311)F2、F3分离群体,将ygl209的叶色突变基因定位于第1染色体着丝粒附近571.6 kb的染色体区段内。对区段内与叶绿体发育有关的基因LOC_Os01g31110序列测定,ygl209突变体中LOC_Os01g31110基因的编码区1390位(位于第5外显子)上碱基由C转换成G,使编码蛋白序列由丙氨酸(Ala)变成了甘氨酸(Gly),推测LOC_Os01g31110即为ygl209的候选基因。
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| 关 键 词： | style="font-family: "Times New Roman" "serif" font-size: 9pt mso-fareast-font-family: 宋体 mso-font-kerning: 1.0pt mso-ansi-language: EN-US mso-fareast-language: ZH-CN mso-bidi-language: AR-SA" Rice" target="_blank">lang="EN-US">Rice ( style="mso-bidi-font-style: Oryza" target="_blank">normal">Oryza sativa L.) Yellow-green leaf mutant Genetic analysis Fine mapping |
| 收稿时间： | 2015-03-21 |
Genetic Analysis and Fine Mapping of Yellow-Green Leaf Mutant ygl209 in Rice |
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| LI Guang-Xian,YAO Fang-Yin,HOU Heng-Jun,SUN Zhao-Wen,JIANG Ming-Song,ZHU Wen-Yin,ZHOU Xue-Biao.Genetic Analysis and Fine Mapping of Yellow-Green Leaf Mutant ygl209 in Rice[J].Acta Agronomica Sinica,2015,41(10):1603-1611. |
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| Authors: | LI Guang-Xian YAO Fang-Yin HOU Heng-Jun SUN Zhao-Wen JIANG Ming-Song ZHU Wen-Yin ZHOU Xue-Biao |
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| Institution: | Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China;3.Agricultural Bureau of Rencheng Distinct, Jining 272000, China |
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| Abstract: | Etiolation mutants of rice play an important role in studies on the photosynthesis, chloroplast development, and chlorophyll metabolism in higher plants. A japonica rice mutant ygl209 with yellow-green leaf was identified from the BC4F3 progeny of the cross between the transgenic variety of Zhongguo 91 and Zhendao 88 with the latter as the recurrent parent. Compared with the wild-type parent Zhendao 88, the contents of chlorophyll a, chlorophyll b and carotenoid decreased dramatically in the mutant ygl209 at the seedling, tillering and heading stages, respectively. In particular, chlorophyll b decreased most significantly. However, there was no significant change in other agronomic traits, such as days to heading, plant height, number of effective panicles per plant, number of grains in main stem panicle, seed setting rate and 1000-grain weight. Genetic analysis showed that the yellow-green leaf trait of the ygl209 mutant was controlled by one pair of recessive nuclear genes. With F2 and F3 segregation populations derived from the cross between ygl209 and Zhendao 88, the YGL209 gene was mapped to the centromere region of chromosome 1, with a physical distance of 571.6 kb. We further analyzed the putative candidate genes in the target region through sequencing. A single base substitution (G1390C) was detected in the coding region of the LOC_Os01g31110 gene, which resulted in a missense mutation (A348G) in its encoded protein. Bioinformatic analysis predicted that the LOC_Os01g31110 gene is related to the chloroplast development in rice. Therefore, LOC_Os01g31110 is likely to be the candidate gene of YGL209. |
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