| 导入LPAAT和KCS基因对油菜种子芥酸含量的影响 |
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| 引用本文： | 陈柳,毛善婧,陆莉,储成才,SONNTAG Karin,王幼平.导入LPAAT和KCS基因对油菜种子芥酸含量的影响[J].作物学报,2006,32(8):1174-1178. |
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| 作者姓名： | 陈柳 毛善婧 陆莉 储成才 SONNTAG Karin 王幼平 |
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| 作者单位： | 1.扬州大学生物科学与技术学院，江苏扬州225009；2中国科学院遗传与发育生物学研究所，北京100101;3.Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Groβ Luesewitz 18190, Germany |
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| 基金项目： | 国家自然科学基金;江苏省六大人才高峰基金 |
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| 摘 要： | 采用农杆菌介导法，以下胚轴为转化受体，将溶血性磷脂酸酰基转移酶（LPAAT）和酮脂酰辅酶A合成酶（KCS）基因导入2种不同基因型（“Drakkar”和“Maplus”）的甘蓝型油菜，共获得247株再生植株，其再生频率分别为15.1%和2.0%。PCR检测和Southern杂交分析证实，外源基因已整合到油菜基因组中，转化频率分别为3.6%和0.7%。种子中芥酸含量因外源基因的导入使零芥酸油菜“Drakkar”提高到10.5%，高芥酸品种“Maplus”提高了5.0%，最高达62.8%。
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| 关 键 词： | 甘蓝型油菜 溶血性磷脂酸酰基转移酶 酮脂酰辅酶A合成酶 芥酸 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2006-01-11 |
Influence of Introducing LPAAT and KCS Genes into Rape on Erucic Acid Content of Seed |
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| CHEN Liu,MAO Shan-Jing,LU Li,CHU Cheng-Cai,SONNTAG Karin,WANG You-Ping.Influence of Introducing LPAAT and KCS Genes into Rape on Erucic Acid Content of Seed[J].Acta Agronomica Sinica,2006,32(8):1174-1178. |
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| Authors: | CHEN Liu MAO Shan-Jing LU Li CHU Cheng-Cai SONNTAG Karin WANG You-Ping |
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| Institution: | 1.College of Bioscience and Biotechnology, Yangzhou 225009, Jiangsu, China；2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;3.Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Groβ Luesewitz 18190, Germany |
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| Abstract: | A protocol for Agrobacterium tumefaciens-mediated transformation of Brassica napus hypocotyls was designed.A strain with a LPAAT(lysophosphatidic acid acyltransferase) gene from Limnanthes douglasii and a KCS(beta-ketoacyl-CoA synthase) gene under control of a napin promoter was co-cultivated with hypocotyls from two genotypes of B.napus,erucic-acid-free cv.'Drakkar' and high-erucic-acid cv.'Maplus'.In total,247 plants were regenerated,with the regeneration percentage of 15.1% for 'Drakkar' and 2.0% for 'Maplus',respectively.Transformants were confirmed by PCR of the LPAAT gene and NAP/KCS expression cassette,and Southern blotting analysis,indicating that the foreign genes were integrated into the genome of rapeseed.The transformation frequencies of B.napus cv.'Drakkar' and 'Maplus' were 3.6% and 0.7%,respectively.Seeds of the transformants showed a changed fatty-acid profile.The content of erucic acid was up to 10.5% in the seeds of transformants of erucic-acid-free cv.'Drakkar' and increased by 5% in the seeds of transformants of cv.'Maplus',compared with the control.The highest content of erucic acid in some individuals of transgenic 'Maplus' was 62.8%. |
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| Keywords: | Brassica napus L LPAAT KCS Erucic acid |
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