Molecular characterization of PRR13 and its tissue-specific expression in rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) |
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| Authors: | Marieke Verleih Alexander Rebl Bernd Köllner Tomá? Korytá? Günter Kotterba Eckhard Anders Klaus Wimmers Tom Goldammer |
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| Institution: | 1.Fachbereich Molekularbiologie,Leibniz-Institut für Nutztierbiologie (FBN),Dummerstorf,Germany;2.Friedrich-Loeffler-Institut (FLI), Institut für Infektionsmedizin,Greifswald, Insel Riems,Germany;3.Landesforschungsanstalt für Landwirtschaft und Fischerei Mecklenburg-Vorpommern (LFA-MV),Institut für Fischerei,Born,Germany |
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| Abstract: | The proline-rich protein 13 (PRR13) is reported to be a key regulator of the resistance to cytostatica by decreasing the copy
number of the proapoptotic gene thrombospondin-1. We isolated and characterized the complete PRR13 gene sequence of rainbow
trout (Oncorhynchus mykiss). The gene comprises four exons and three introns, the latter of comparatively short lengths (100–811 bp). The full-length
PRR13 cDNA consists of 1,101 nucleotides, including an open reading frame of 563 bp, which is predicted to encode a 187 amino
acid protein with a molecular mass of 18.8 kDa. A continuous stretch of ten serine residues at the C-terminus is highly conserved
and characteristic for vertebrate PRR13, but not for other known proline-rich proteins. Phylogenetic analyses suggest a clear
separation of teleostean PRR13 proteins and those from mammalian and reptilian species. Comparison of the tissue-specific
PRR13 mRNA abundance in two strains of the rainbow trout coastal form (TCO Steelhead II-WA vs. BORN Steelhead II-Germany)
revealed an increased expression in the BORN trout in nearly all examined tissues. The major expression differences were detected
in gill (2.29-fold) and in liver tissue (2.16-fold). Hence, the increased PRR13 expression in BORN trout might cause improved
protection from natural cytostatica and therefore support our assumption that PRR13 is a candidate gene possibly involved
in the varying ability of the two rainbow trout strains to handle environmental stress under local conditions of the Southern
Baltic. |
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