| 马立克氏病病毒VP22基因siRNA筛选体系的构建 |
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| 引用本文： | 缪德年,王秀花,姜法铭,陈溥言,樊生超.马立克氏病病毒VP22基因siRNA筛选体系的构建[J].南京农业大学学报,2006,29(4):143-145. |
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| 作者姓名： | 缪德年 王秀花 姜法铭 陈溥言 樊生超 |
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| 作者单位： | 1. 上海市农业科学院畜牧兽医研究所,上海,201106
2. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 上海市青年科技启明星计划 |
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| 摘 要： | 将马立克氏病病毒（MDV）超强毒株VP22基因插入真核表达载体pEGFP-C1中EGFP基因的下游，构建EGFP-VP22融合基因真核表达载体pEGFP-VP22。将其转染至中国仓鼠卵巢细胞（CHO-k1）中，通过荧光显微镜观察EGFP的表达情况，并用RT-PCR检测VP22基因的表达。检测证实EGFP和VP22在CHO细胞中均得到了有效表达。
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| 关 键 词： | 马立克氏病病毒 VP22基因 筛选体系 小干扰RNA |
| 文章编号： | 1000-2030(2006)04-0143-03 |
| 收稿时间： | 12 28 2005 12:00AM |
| 修稿时间： | 2005年12月28 |
Construction of screening system for siRNA targeted to VP22 gene of MDV |
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| MIAO De-nian,WANG Xiu-hua,JIANG Fa-ming,CHEN Pu-yan,FAN Sheng-chao.Construction of screening system for siRNA targeted to VP22 gene of MDV[J].Journal of Nanjing Agricultural University,2006,29(4):143-145. |
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| Authors: | MIAO De-nian WANG Xiu-hua JIANG Fa-ming CHEN Pu-yan FAN Sheng-chao |
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| Institution: | 1. Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 2. Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | pEGFP-VP22, an eukaryotic expression vector containing EGFP-VP22, was constructed by inserting VP22 gene of MDV RB1B strain into downstream of EGFP gene in eukaryotic expression vector pEGFP-C1. Then the pEGFP-C1 and pEGFP-VP22 were transfected into CHO-k1 cells with siPORT^TM XP-1. Expression of EGFP and VP22 were detected by reflected fluorescence microscope screen and RT-PCR respectively. Digestion of the recombinant plasmid showed that VP22 gene was successfully inserted into pEGFP-C1. Both EGFP and VP22 were effectively expressed in CHO cells transfected with the recombinant plasmid pEGFP-VP22, which build the basis for effective evaluation of RNAi directed by siRNA through green fluorescent protein. |
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| Keywords: | MDV VP22 gene screening system siRNA |
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