A new disease of mung bean caused by Botrytis cinerea |
| |
| Institution: | 1. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China;2. Chongqing Academy of Agricultural Sciences, Chongqing, 402160, PR China;1. Department of Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa;2. Genomics Research Institute, University of Pretoria, South Africa;3. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa;4. Department of Plant Pathology, University of Arkansas, Fayetteville, AR, USA;1. ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur, India;2. Banda University of Agriculture and Technology, Banda, India;1. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, USA;2. USDA-ARS, West Lafayette, IN 47909, USA;1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China;2. Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, China;3. College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;4. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, China;1. Institute of Plant Biology and Biotechnology, University of Münster, Schlossplatz 8, 48143 Münster, Germany;2. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Straße 24, 3430 Tulln an der Donau, Austria |
| |
| Abstract: | Gray mold caused by Botrytis cinerea, a ubiquitous and destructive plant pathogen, is an important disease of various crops including orchard crops, vegetables and ornamental plants. In June 2012, symptoms similar to gray mold were observed on mung bean (Vigna radiata) plants in Yongchuan, Chongqing, China. To confirm the causal agent, the pathogen was isolated and six fungal isolates were identified based on morphological and molecular characterization. The six isolates showed similar morphology to Botrytis cinerea. The rDNA-ITS sequences of the six isolates showed 99% identity to reported B. cinerea strains. The two specific primer pairs for B. cinerea, C729+/C729? and Bc108+/Bc563?, produced target fragments of 700-bp and 450-bp, respectively, in all six isolates, and their sequences displayed 99–100% identity to known B. cinerea strains. All of the isolates harbored two transposable elements of B. cinerea, Flipper and Boty, which indicated that they all belonged to the transposa group. Subsequently, pathogenicity tests demonstrated the pathogen was virulent not only on mung bean but also on other legume crops, including Phaseolus vulgaris, Vigna unguiculata, Vigna angularis, Pisum sativum, Vicia faba, and Glycine max. The results of morphological and molecular identification combined with pathogenicity tests confirmed that the pathogen isolated from mung bean was B. cinerea. To our knowledge, this is the first report of B. cinerea causing gray mold on mung bean in the world. |
| |
| Keywords: | Gray mold disease |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
