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Elimination of <Emphasis Type="Italic">Plum pox virus</Emphasis> through in vitro thermotherapy and shoot tip culture compared to conventional heat treatment in apricot cultivar Bebecou |
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| Authors: | Georgios C Koubouris Varvara I Maliogka Kostadinos Efthimiou Nikolaos I Katis Miltiadis D Vasilakakis |
| Institution: | (1) Pomology Lab, School of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece;(2) Plant Pathology Lab, School of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece;(3) Present address: Laboratory of Genetic Improvement, Physiology and Olive Cultivation Techniques, Institute of Olive Tree and Subtropical Plants of Chania, NAGREF, Agrokipio, 73100 Chania, Greece |
| Abstract: | The effectiveness of in vitro thermotherapy (explants at 35–37°C for 20 days) in obtaining propagating material of apricot (Prunus armeniaca L.) cultivar Bebecou that is free of Plum pox virus (PPV) was compared to a conventional heat treatment technique (potted plants at 30–35°C for 8 weeks). An improved protocol for in vitro thermotherapy was combined with shoot tip culture. The protocol is 5.8 times more effective than conventional thermotherapy while taking half the time and is likely to have a high commercial impact for nurseries. |
| Keywords: | Certification ELISA IC-RT-PCR Plum pox virus Propagating material Prunus armeniaca L |
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