| 植物根际促生细菌(PGPR)分离筛选与鉴定 |
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| 引用本文： | 杨蓉,房世杰,杨文琦,侯敏,詹发强,侯新强,张慧涛,龙宣杞,崔卫东.植物根际促生细菌(PGPR)分离筛选与鉴定[J].新疆农业科学,2011,48(12):2337-2342. |
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| 作者姓名： | 杨蓉 房世杰 杨文琦 侯敏 詹发强 侯新强 张慧涛 龙宣杞 崔卫东 |
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| 作者单位： | 新疆农业科学院微生物应用研究所,乌鲁木齐830091;新疆农业大学农学院,乌鲁木齐830052;新疆农业科学院科研管理处,乌鲁木齐,830091;新疆农业科学院微生物应用研究所,乌鲁木齐,830091 |
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| 基金项目： | 新疆维吾尔自治区科技计划项目“设施农业多价菌剂的研制”(201010103);新疆乌鲁木齐市科技计划项目“设施农业复合菌剂的研制”(Y111210003) |
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| 摘 要： | 目的]从不同作物根际土壤中筛选对黄瓜和番茄有明显促生效应的植物根际促生细菌(PGPR).方法]从番茄、黄瓜、茄子、辣椒四种蔬菜作物的根际土壤中进行分离,测定菌株的促生能力,获得优势广适菌株,再根据菌体形态、培养特征及16S rDNA部分序列分析进行鉴定.结果]共分离得到24株细菌菌株,经平皿初筛,分别筛选出对黄瓜幼苗、番茄幼苗生长有显著促生作用的细菌11和5株,再经营养钵促生实验筛选出2株对黄瓜、番茄幼苗均具有显著促生作用的PGPR菌,经16S rDNA序列分析,分别为芽孢杆菌属(Bacillus)和布克霍尔德氏菌属(Burkholderia).结论]所筛选细菌对黄瓜和番茄幼苗有显著促生作用,为进一步构建PGPR广适菌群提供了菌株资源.
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| 关 键 词： | 植物根际促生菌 分离 筛选 鉴定 |
| 收稿时间： | 2011-12-25 |
Sieving and Identification of Plant Growth-Promoting Rhizobacteria |
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| YANG Rong
,
FANG Shi-jie
,
YANG Wen-qi
,
HOU Min
,
ZHAN Fa-qiang
,
HOU Xin-Qiang
,
ZHANG Hui-tao
,
LONG Xuan-qi
,
CUI Wei-dong.Sieving and Identification of Plant Growth-Promoting Rhizobacteria[J].Xinjiang Agricultural Sciences,2011,48(12):2337-2342. |
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| Authors: | YANG Rong FANG Shi-jie YANG Wen-qi HOU Min ZHAN Fa-qiang HOU Xin-Qiang ZHANG Hui-tao LONG Xuan-qi CUI Wei-dong |
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| Institution: | 1(1.Research Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;2.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China;3.Xinjiang Academy of Agricultural Sciences,Urumqi 830091) |
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| Abstract: | 【Objective】 The purpose of this project was to screen out effective plant growth-promoting rhizobacteria strains(PGPR) for cucumber and tomato from different vegetable rhizosphere soil.【Method】PGPR strains were isolated from rhizosphere soil of tomato,cucumber,eggplant and capsicum/chilli based on growth-promoting effect investigation by petri dish preliminary screening and nutrition pot experiment.The selected efficient and broad adaptive strains were then identified by morphological,physiochemical,and 16S rDNA analysis.【Result】24 strains of PGPR were screened primarily,and 11 strains and 5 strains of them could obviously promote the growth of cucumber and tomato respectively.2 strains of them were proved to be efficient for both vegetable by nutrition pot experiment,and were subsequently identified as Bacillus sp.and Burkholderia sp.respectively.【Conclusion】The 2 screened strains could remarkably promote the growth of cucumber and tomato seedlings which could be strain material for broad adaptive PGPR library. |
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| Keywords: | plant growth-promoting rhizobacteria(PGPR) isolation screening identification |
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