| 仙居鸡染色体G带和Ag-NORs的研究 |
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| 引用本文： | 陈国宏,李碧春,徐琪,张学余,吴信生,刘莉,嵇宝华.仙居鸡染色体G带和Ag-NORs的研究[J].畜牧兽医学报,2004,35(2):141-145. |
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| 作者姓名： | 陈国宏 李碧春 徐琪 张学余 吴信生 刘莉 嵇宝华 |
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| 作者单位： | 1. 扬州大学畜牧兽医学院动科系,扬州,225009
2. 中国农业科学院家禽研究所,扬州,225003 |
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| 基金项目： | 国家高新技术发展计划项目(863 2001AA243082)，国家自然科学基金资助项目(30170673) |
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| 摘 要： | 运用改进的鸡外周血淋巴细胞培养——空气干燥法，分析了仙居鸡染色体G带和Ag—NORs。G带研究结果表明：前10对大型染色体可分为30个区，144条带，同时对G带带型特征进行了具体描述，并绘制了G带模式图。Ag—NORs处理发现：仙居鸡的Ag—NORs常分布于1p、3p、4p、Zp、2q上，均数为3．26，众数为3。不同个体间以及同一个体的不同细胞间。Ag—NORs数目存在多态性；此外研究还发现Ag—NORs联合现象。
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| 关 键 词： | 仙居鸡 染色体 G带 Ag-NORs 血淋巴细胞 细胞培养 空气干燥法 |
| 文章编号： | 0366-6964(2004)02-0141-05 |
Studies on Chromosome G Band and Ag-NORs of Xianju Chicken |
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| CHEN Guo- hong,LI Bi- chun,XU Qi,ZHANG Xue- yu,WU Xin- sheng,LIU Li,JI Bao- hua.Studies on Chromosome G Band and Ag-NORs of Xianju Chicken[J].Acta Veterinaria et Zootechnica Sinica,2004,35(2):141-145. |
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| Authors: | CHEN Guo- hong LI Bi- chun XU Qi ZHANG Xue- yu WU Xin- sheng LIU Li JI Bao- hua |
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| Institution: | CHEN Guo- hong~1,LI Bi- chun~1,XU Qi~1,ZHANG Xue- yu~2,WU Xin- sheng~1,LIU Li~1,JI Bao- hua~1 |
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| Abstract: | In this study, the chromosome slides were made by improved peripheral blood lymphocyte culture- drying naturally method, and analyzed G- band patterns, Ag- NORs patterns of Xianju chickens. The results were as follow: Xianju chickens' G- band of the first ten pairs chromosomes was divided into 30 (regions) and 144 bands, its character was described and its idiogram was depicted. Xianju chickens' Ag- NORs were located on 1p, 3p, 4p, Zp and 2q, the mean value per cell was 3.26 and the mode was 3. The size, number and distribution between different individuals and of the same individual were polymorphic and associations of Ag- NORs were found . |
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| Keywords: | Chicken Chromosome G- Band Ag- NORs |
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