Acute effects of benzo[a]pyrene on liver phase I and II enzymes,and DNA damage on sea bream <Emphasis Type="Italic">Sparus aurata</Emphasis> |
| |
| Authors: | M Banni Z Bouraoui J Ghedira C Clerandeau H Guerbej J F Narbonne H Boussetta |
| |
| Institution: | (1) Laboratoire de Biochimie et de Toxicologie Environnementale, Institut Supérieur Agronomique, Chott-Mariem, Sousse, Tunisia;(2) Laboratoire de Physico-Toxico Chimie des Systèmes Naturels, Université Bordeaux I, Bordeaux, France;(3) Laboratoire de Biotechnologie Marine, INSTM, Monastir, 5000 Monastir, Tunisia |
| |
| Abstract: | In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons
benzoa]pyrene (Ba]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of Ba]P (20 mg kg−1) for 6, 12, 24, and 48 h. Ba]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS)
after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel
electrophoresis comet assay. Ba]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up
to 5.55 ± 0.67 μg g−1 dry weight after only 6 h exposure and 4.67 ± 0.68 μg g−1 dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower
but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after
48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to
12.17 %DNA in the tail. |
| |
| Keywords: | Acute exposure Benzo[a]pyrene EROD activity GST activity DNA damage |
| 本文献已被 SpringerLink 等数据库收录！ |
|
