| 叶面施硒对黑豆硒吸收转运特征及籽粒硒形态的影响 |
| |
| 引用本文： | 杜小平,刘乔斐,夏曾润,贺博,卜贤盼,祁蒙,唐德剑,佘婷婷.叶面施硒对黑豆硒吸收转运特征及籽粒硒形态的影响[J].核农学报,2022,36(11):2266-2274. |
| |
| 作者姓名： | 杜小平 刘乔斐 夏曾润 贺博 卜贤盼 祁蒙 唐德剑 佘婷婷 |
| |
| 作者单位： | 1安康市富硒产品研发中心/农业农村部富硒产品开发与质量控制重点实验室/富硒食品开发国家地方联合工程实验室,陕西 安康 7250002安康学院现代农业与生物科技学院,陕西 安康 725000 |
| |
| 基金项目： | 陕西省科协企业创新争先青年人才托举计划项目(2021-1-2);陕西省科技厅重点研发计划项目(2021NY-043);陕西省技术创新引导计划项目(2022QFY09-03);安康市科学技术研究发展计划项目(AK2020-FX01-8) |
| |
| 摘 要： | 为了明确叶面喷施亚硒酸钠对黑豆硒吸收转运特征及籽粒硒形态的影响,采用小区试验在黑豆结荚兴盛期进行一次性叶面喷施亚硒酸钠(施硒量分别为0、6、12、18和24 mg·m-2)处理,分析比较不同浓度亚硒酸钠处理下黑豆产量、各器官硒含量、籽粒有机硒转化率和品质性状的差异。结果表明,各施硒水平对黑豆各器官总硒含量均有提升作用,在24 mg·m-2硒处理时各器官总硒含量最大,根、茎、荚和籽粒硒含量分别较对照提高了8.74、8.37、9.85和65.21倍,硒处理后各器官总硒含量呈现籽粒>根≈荚>茎的特点。籽粒中硒以有机硒形态存在,且有机硒转化率随着亚硒酸钠浓度的增加呈下降趋势,在0、6、12、18和24 mg·m-2施硒处理时,有机硒含量分别为0.071 4、1.101 8、1.510 6、1.811 1和2.093 5 mg·kg-1,有机硒转化率分别为94.52%、84.81%、70.30%、60.11%和52.43%。叶面喷施亚硒酸钠显著提高了黑豆籽粒蛋白质含量和分离蛋白硒含量,但对脂肪含量的影响较小,在24 mg·m-2硒处理时蛋白质含量和分离蛋白硒含量分别较对照增加了2.4个百分点和117.35倍,分离蛋白硒含量占有机硒和总硒含量的比例分别为90.29%和47.39%。适宜浓度亚硒酸钠叶面喷施对黑豆产量具有促进作用,但浓度过高导致增产效果减弱。综上,叶面喷施亚硒酸钠可提高黑豆各器官总硒含量,但黑豆籽粒有机硒转化率随喷施浓度的增加呈下降趋势,且黑豆籽粒有机硒主要以蛋白质形式存在。本研究结果为应用外源硒强化技术生产富硒黑豆提供了科学依据。
|
| 关 键 词： | 黑豆 亚硒酸钠 吸收 转运 叶面喷施 |
| 收稿时间： | 2022-04-02 |
Effects of Selenium Foliar Spray on Selenium Uptake and Translocation Characteristics and Seed Selenium Speciation in Black Beans |
| |
| DU Xiaoping,LIU Qiaofei,XIA Zengrun,HE Bo,BU Xianpan,QI Meng,TANG Dejian,SHE Tingting.Effects of Selenium Foliar Spray on Selenium Uptake and Translocation Characteristics and Seed Selenium Speciation in Black Beans[J].Acta Agriculturae Nucleatae Sinica,2022,36(11):2266-2274. |
| |
| Authors: | DU Xiaoping LIU Qiaofei XIA Zengrun HE Bo BU Xianpan QI Meng TANG Dejian SHE Tingting |
| |
| Institution: | 1Ankang R&D Center for Se-enriched Products/Key Laboratory of Se-enriched Products Development and Quality Control, Ministry of Agriculture and Rural Affairs/National-Local Joint Engineering Laboratory of Se-enriched Food Development, Ankang, Shaanxi 7250002School of Modern Agriculture and Biotechnology, Ankang University, Ankang, Shaanxi 725000 |
| |
| Abstract: | In order to clarify effect of foliar application of sodium selenite on the selenium (Se) uptake and translocation characteristics and seed Se speciation in black beans, a plot experiment with five treatments (0, 6, 12, 18 and 24 mg·m-2 Se in sodium selenite) was conducted by a one-time foliar spray of sodium selenite in black beans at the poding stages to analyze and compare the differences in yield, Se content of organs, conversion rate of organic Se in seeds, and quality traits in black soybean treated with different concentrations of sodium selenium. The results showed that the total Se content of all organs in black beans was enhanced by foliage spraying at each level. The total Se content of all organs reached the maxium after treated with Se at 24 mg·m-2, and the selenium content in roots, stems, pods and seeds was increased by 8.74, 8.37, 9.85 and 65.21 times, respectively, compared with the control. The total Se concentrations in different black bean tissues after Se treatment showed the characteristics of seed> root≈ pod> stem. Se in black bean seeds mainly is present as organic Se, and the conversion rate of organic Se was decreased with the increase of sodium selenite concentration. The organic Se concentrations were 0.071 4, 1.101 8, 1.510 6, 1.811 1 and 2.093 5 mg·kg-1, and the organic Se conversion rates were 94.52%, 84.81%, 70.30%, 60.11% and 52.43%, respectively, at the spraying treatment of 0, 6, 12, 18 and 24 mg·m-2. Spraying sodium selenite in leaves significantly enhanced protein synthesis, but had little effect on fat content in black beans. Compared with control, protein content and Se concentration in isolated protein increased by 2.4 percentage points and 117.35 times, and the Se concentration in isolated protein accounted for 90.29% and 47.39% of organic Se and total Se, respectively. Foliage spraying with appropriate concentration of sodium selenite could promote the yield of black bean, but the effect was weakened if the concentration was too high. In conclusion, foliar application of sodium selenite can increase the total Se content of various organs in black bean, but the conversion rate of organic Se in black bean seed decreased with the increase of spraying concentration. The organic Se in black bean seeds mainly exists in the form of protein. This study provides a scientific basis for the production of Se-rich black beans through strengthening technology of exogenous selenium fortification. |
| |
| Keywords: | black soybean sodium selenite uptake translocation foliar spraying |
|
| 点击此处可从《核农学报》浏览原始摘要信息 |
| 点击此处可从《核农学报》下载免费的PDF全文 |
|
