| 60Co-γ辐照模式下玉米中赤霉烯酮和品质的变化及对绵羊定时人工授精的影响 |
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| 引用本文： | 李心海,朱广琴,高峰,张林吉,任士飞,王岩,崔燕.60Co-γ辐照模式下玉米中赤霉烯酮和品质的变化及对绵羊定时人工授精的影响[J].核农学报,2022,36(11):2258-2265. |
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| 作者姓名： | 李心海 朱广琴 高峰 张林吉 任士飞 王岩 崔燕 |
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| 作者单位： | 1甘肃农业大学动物医学院,甘肃 兰州 7300702徐州生物工程职业技术学院,江苏 徐州 2211513江苏汉羊牧业生态科技有限公司,江苏 徐州 221151 |
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| 基金项目： | 江苏现代农业产业技术体系建设专项资金(JATS[2021]051);徐州市科技项目(KC20048) |
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| 摘 要： | 为研究经赤霉烯酮(ZEN)自然污染与60Co-γ辐照处理后的玉米对绵羊定时人工授精中澳洲白种公羊、湖羊母羊的生殖性能的影响,本试验选取含ZEN玉米样本,分别用0、3、5、8、10 kGy剂量的60Co-γ辐照,并测定辐照后玉米主要营养成分变化。然后分别将未污染玉米(Ⅰ对照组)、经10 kGy 60Co-γ辐照的污染玉米(试验Ⅱ组)、污染玉米(试验Ⅲ组)饲喂定时人工授精的绵羊,检测澳洲白种公羊的精液质量,测定湖羊母羊的繁殖性能指标。结果表明,未经60Co-γ辐照的玉米含ZEN 2 153 μg·kg-1,在10 kGy 60Co-γ辐照剂量下,玉米中ZEN降解率为83.7%;当60Co-γ辐照剂量低于10 kGy时,玉米淀粉表面结构无变化,主要营养成分无显著变化。试验Ⅱ组澳洲白种公羊的采精量、精子活力、精子密度与试验Ⅲ组相比显著升高(P<0.05),与Ⅰ对照组相比无显著差异(P>0.05);试验Ⅲ组澳洲白种公羊第30天精子畸形率与试验Ⅱ组、Ⅰ对照组相比均显著升高(P<0.05),显微镜下观察可见双尾精子、双头精子、无尾精子等现象,其中双尾精子头部、尾部不完整;试验Ⅱ组湖羊母羊同期发情率、受胎率、产羔率和羔羊始重等指标与试验Ⅲ组相比显著升高(P<0.05),与Ⅰ对照组无显著差异(P>0.05)。综上,使用经10 kGy 60Co-γ辐照后ZEN含量低于2 153 μg·kg-1的玉米饲喂繁育绵羊,对绵羊定时人工授精无不利影响。本研究结果为60Co-γ辐照模式下玉米中赤霉烯酮和品质的变化对绵羊定时人工授精的影响研究提供了理论依据。
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| 关 键 词： | 玉米 玉米赤霉烯酮 60Co-γ辐照 品质 绵羊 |
| 收稿时间： | 2021-11-16 |
Changes of Zearalenone and Quality in Maize Under 60Co-γ Irradiation and Its Effect on Timed Artificial Insemination of Sheep |
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| LI Xinhai,ZHU Guangqin,GAO Feng,ZHANG Linji,REN Shifei,WANG Yan,CUI Yan.Changes of Zearalenone and Quality in Maize Under 60Co-γ Irradiation and Its Effect on Timed Artificial Insemination of Sheep[J].Acta Agriculturae Nucleatae Sinica,2022,36(11):2258-2265. |
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| Authors: | LI Xinhai ZHU Guangqin GAO Feng ZHANG Linji REN Shifei WANG Yan CUI Yan |
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| Institution: | 1College of Veterinary Medicine,Gansu Agricultural University, Lanzhou, Gansu 7300702Xuzhou Vocational College of Bioengineering, Xuzhou, Jiangsu 2211513Jiangsu Hanyang Sheep Animal Husbandry Ecological Technology Co, LTD, Xuzhou, Jiangsu 221151 |
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| Abstract: | In order to study the effect of maize after natural pollution by gibberellin (ZEN) and 60Co-γ irradiation on the reproductive performance of Australian white rams and Hu sheep ewes in timing artificial insemination of sheep. In this experiment, maize samples containing ZEN were selected and irradiated with 60Co-γ at doses of 0, 3, 5, 8 and 10 kGy, respectively, and the changes of main nutrients in maize after irradiation were determined. Then, unpolluted maize (control group Ⅰ), contaminated maize irradiated by 10 kGy 60Co-γ (test group Ⅱ) and contaminated corn (test group Ⅲ) were fed to sheep with regular artificial insemination. The semen quality of Australian white rams was tested, and the reproductive performance index of lake sheep ewes was measured. The results showed that maize without 60Co-γ irradiation contained 2 153 μg·kg-1 ZEN, and the degradation rate of ZEN in maize was 83.7% at 10 kGy 60Co-γ irradiation dose.When the irradiation dose of 60Co-γ was lower than 10 kGy, the surface structure of maize starch did not change, and the main nutrients did not change significantly. The semen volume, sperm motility and sperm density in group Ⅱ were significantly higher than those in group Ⅲ (P<0.05). On the 30th day, the sperm abnormality rate of Australian white rams in Group Ⅲ was significantly higher than that in Group Ⅱ and Group Ⅰ (P<0.05). In conclusion, corn with ZEN content less than 2 153 μg·kg-1 after 10 kGy 60Co-γ irradiation had no adverse effect on sheep timing artificial insemination. This study provides a theoretical basis for the effect of changes in zearalenone and quality in maize on sheep timing artificial insemination under 60Co-γ irradiation. |
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| Keywords: | corn zearalenone 60Co-γ irradiation quality sheep |
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