| 皂荚皂苷元制备及纯化工艺研究 |
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| 引用本文： | 刘艳萍,王莹,张静,范定臣,刘佳佳,赵俊杰,薛刚.皂荚皂苷元制备及纯化工艺研究[J].中国农学通报,2022,38(28):143-149. |
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| 作者姓名： | 刘艳萍 王莹 张静 范定臣 刘佳佳 赵俊杰 薛刚 |
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| 作者单位： | 1.河南省林业科学研究院,郑州 450008;2.南阳理工学院河南省工业微生物资源与发酵技术重点实验室,河南南阳 473004;3.河南投资集团有限公司,郑州 450008;4.河南省弘富林药业有限公司,河南内乡 474350 |
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| 基金项目： | 河南省基本科研业务费项目“皂荚皂苷提取及皂苷元制备工艺”(2021JB02002) |
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| 摘 要： | 为优化皂荚皂苷酸解制备皂苷元的工艺,以皂荚皂苷粗品为原料,考察酸浓度、酸解时间、酸解温度、料液比和酸种类对皂荚皂苷酸解转化成皂荚皂苷元的影响。结果表明,最佳酸解工艺为盐酸浓度4 mol/L、酸解时间4 h、酸解温度80℃、料液比1:30(m:v),采用优化后的酸解条件能够得到含量在65%左右的皂荚皂苷元产品。再通过有机溶剂萃取法和重结晶法对酸水解后的皂苷元进行纯化,确定最佳的萃取剂为三氯乙烯,料液比为1:20(m:v),萃取时间在90 min内变化不大,萃取1次,采用60%乙醇重结晶,得到的总皂苷元纯度可达76.31%。
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| 关 键 词： | 皂荚皂苷 皂苷元 酸解 有机溶剂萃取 重结晶 |
| 收稿时间： | 2021-11-15 |
Study on the Preparation and Purification Process of Sapogenin from Gleditsia sinensis |
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| LIU Yanping,WANG Ying,ZHANG Jing,FAN Dingchen,LIU Jiajia,ZHAO Junjie,XUE Gang.Study on the Preparation and Purification Process of Sapogenin from Gleditsia sinensis[J].Chinese Agricultural Science Bulletin,2022,38(28):143-149. |
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| Authors: | LIU Yanping WANG Ying ZHANG Jing FAN Dingchen LIU Jiajia ZHAO Junjie XUE Gang |
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| Institution: | 1.Henan Academy of Forestry, Zhengzhou 450008;2.Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology, Nanyang Institute of Technology, Nanyang, Henan 473004;3.Henan Investment Group Limited Company, Zhengzhou 450008;4.Henan Hongfu Forestry and Pharmaceutical Co., Ltd., Neixiang, Henan 474350 |
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| Abstract: | To optimize the preparation process of sapogenin from Gleditsia sinensis saponin by acid hydrolysis, the effects of acid concentration, acidolysis time, acidolysis temperature, material-to-liquid ratio and acid types on the conversion of saponin into sapogenin by acid hydrolysis were investigated. The results showed that the optimum conditions were as follows: hydrochloric acid concentration of 4 mol/L, hydrolysis time of 4 h, hydrolysis temperature of 80℃, solid-liquid ratio of 1:30 (m:v), and the content of Gleditsia sinensis sapogenin product was about 65%. The sapogenin after acid hydrolysis was purified by organic solvent extraction and recrystallization. The best extraction agent was trichloroethylene, the solid-liquid ratio was 1:20 (m:v), and extraction time did not change much within 90 minutes. After one extraction and recrystallization with 60% ethanol, the purity of total sapogenin could reach 76.31%. |
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| Keywords: | saponin of Gleditsia sinensis sapogenin acidolysis organic solvent extraction recrystallization |
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