| 杉木应压木形成中的显微特征及主要代谢成分变化 |
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| 引用本文： | 杜明秋,钟珊丽,林二培,黄华宏.杉木应压木形成中的显微特征及主要代谢成分变化[J].核农学报,2022,36(11):2307-2315. |
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| 作者姓名： | 杜明秋 钟珊丽 林二培 黄华宏 |
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| 作者单位： | 浙江农林大学亚热带森林培育国家重点实验室,浙江 杭州 311300 |
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| 基金项目： | 浙江省农业新品种选育项目(2021C02070-8);“十三五”国家重点研究计划林业资源培育及高效利用技术创新重点专项(2017YFD0600201) |
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| 摘 要： | 为了解析主要代谢成分与杉木(Cunninghamia lanceolata)应压木形成的关系,以3年生直立杉木苗为研究对象,与垂直方向倾斜45°放置30、60和90 d后观察其生长性状并测定木质纤维素含量,使用气相色谱-质谱联用仪(GC-MS)对木质部代谢成分进行鉴定和解析。解剖发现应压木木质部管胞发生变形;化学成分测定发现总木质素和半纤维素含量增加,纤维素含量降低;GC-MS检测出应压木、对应木和直立木中共有成分20种,其中有机酸7种、单糖3种、二糖2种、醇类3种、氨基酸2种、酚类和糖苷类各1种;主成分分析结合显著性检验发现,3个时期应压木和直立木中有11种共有差异代谢物,其中乙醇酸、甘氨酸、核糖酸和棕榈酸在应压木中的含量显著低于对应木和直立木,苹果酸、松醇、肌醇、半乳糖和蔗糖在应压木中的含量显著高于对应木和直立木。本试验结果从代谢角度解释了杉木应压木形成中的生物学变化,可为揭示木材形成的分子机制提供新途径,并为高品质木材的定向培育和木材功能性改良提供理论依据。
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| 关 键 词： | 应压木 杉木 有机酸类 糖类 气相色谱-质谱法(GC-MS) |
| 收稿时间： | 2021-11-01 |
Anatomy Characteristics and Study of Alterations of Key Metabolic Components in Cunninghamia lanceolata During Compression Wood Formation |
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| DU Mingqiu,CHONG Sunli,LIN Erpei,HUANG Huahong.Anatomy Characteristics and Study of Alterations of Key Metabolic Components in Cunninghamia lanceolata During Compression Wood Formation[J].Acta Agriculturae Nucleatae Sinica,2022,36(11):2307-2315. |
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| Authors: | DU Mingqiu CHONG Sunli LIN Erpei HUANG Huahong |
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| Institution: | Zhejiang Griculture and Forestry University, Hangzhou, Zhejiang 311300 |
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| Abstract: | To explore the relationship between the major metabolic components and the compression wood formation of Cunninghamia lanceolata, three-year-old erect seedlings of C. lanceolata were selected as materials for the research, observe its growth characteristics after being placed at an inclination of 45° to the vertical for 30, 60 and 90 days, and determine the lignocellulose content, gas chromatography-mass spectrometry(GC-MS)was used to identify and analyze the metabolic components of the xylem. Tracheid anatomy revealed that the cell walls of the compression wood were deformed. Chemical composition analysis showed that the content of total lignin and hemicellulose increased, and the content of cellulose decreased. The GC-MS results identified 20 components in compression wood(CW), opposite wood(OW)and control/straight wood(CK). These include seven organic acids, three monosaccharides, two disaccharides, three types of alcohol, two types of amino acids, one phenol and one glycoside. Principal component analysis and significance test identified 11 common metabolites between different treatments and time intervals. Among them, the content of glycolic acid, glycine, ribonic acid and palmitic acid in CW was significantly lower than that in OW and CK. In addition, galactose and sucrose content in CW was much higher than that in OW and CK. The experimental results give a new insight to reveal compression wood formation of C. lanceolata at a metabolic level. Understanding the molecular mechanism in compression wood formation benefits the directed breeding of high-quality wood species. This study provides theoretical basis for functional improvement of wood. |
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| Keywords: | compression wood Cunninghamia lanceolata organic acids sugars gas chromatography-mass spectrometry(GC-MS) |
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