| 灌浆期高温胁迫下外源水杨酸对小麦旗叶抗氧化生理特性的影响 |
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| 引用本文： | 樊永惠,李宇星,马亮亮,吕钊彦,武倩倩,张文静,马尚宇,黄正来.灌浆期高温胁迫下外源水杨酸对小麦旗叶抗氧化生理特性的影响[J].核农学报,2022,36(9):1878-1886. |
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| 作者姓名： | 樊永惠 李宇星 马亮亮 吕钊彦 武倩倩 张文静 马尚宇 黄正来 |
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| 作者单位： | 1安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,安徽 合肥 2300362安徽农业大学园艺学院,安徽 合肥 230036 |
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| 基金项目： | 安徽省自然科学基金项目(2008085QC118);国家自然科学基金联合基金项目(U19A2021);安徽农业大学稳定和引进人才科研项目(yj2018-38);安徽农业大学青年基金重点项目(03087060) |
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| 摘 要： | 为探讨外源水杨酸(SA)对缓解小麦花后高温伤害的作用,本研究于2019—2020年进行盆栽试验,以扬麦18号为材料,在田间增温棚中进行花后15~19 d的高温胁迫处理,比较不同时期预喷施外源SA处理下小麦的抗氧化生理特性。结果表明,高温胁迫处理导致丙二醛(MDA)和过氧化氢(H2O2)含量增加,旗叶超氧阴离子( )产生速率加快,小麦细胞膜脂过氧化程度加剧,对小麦旗叶造成损害;喷施外源SA后,灌浆期小麦旗叶超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)等抗氧化酶活性有所提高,抗逆指标苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)活性提高,表明外源SA增强了小麦对高温环境的抵抗能力;不同时期喷施外源SA后,开花期预喷施SA处理相对其他处理缓解高温胁迫伤害效果最好。综上,喷施外源SA可通过提高小麦旗叶抗氧化酶和抗逆酶的活性缓解灌浆期高温胁迫对小麦旗叶的损伤程度,且开花期喷施效果较佳。本研究结果为减轻高温对小麦生产的危害提供了新的技术途径。
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| 关 键 词： | 小麦 水杨酸 灌浆期 旗叶 氧化特性 |
| 收稿时间： | 2021-09-08 |
Effects of Exogenous Salicylic Acid on Antioxidant Physiological Characteristics of Wheat Flag Leaves Under High Temperature Stress at Grain Filling Stage |
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| FAN Yonghui,LI Yuxing,MA Liangliang,LYU Zhaoyan,WU Qianqian,ZHANG Wenjing,MA Shangyu,HUANG Zhenglai.Effects of Exogenous Salicylic Acid on Antioxidant Physiological Characteristics of Wheat Flag Leaves Under High Temperature Stress at Grain Filling Stage[J].Acta Agriculturae Nucleatae Sinica,2022,36(9):1878-1886. |
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| Authors: | FAN Yonghui LI Yuxing MA Liangliang LYU Zhaoyan WU Qianqian ZHANG Wenjing MA Shangyu HUANG Zhenglai |
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| Institution: | 1College of Agriculture, Anhui Agricultural University/Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai, Ministry of Agriculture, Hefei, Anhui 2300362College of Horticulture, Anhui Agricultural University, Hefei, Anhui 230036 |
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| Abstract: | A pot experiment was conducted from 2019 to 2020 in order to explore the relationship between exogenous salicylic acid (SA) and the alleviation of high temperature injury after athesis in wheat. To investigate the mechanism underlying the SA-mediated alleviation of the heat stress damage to wheat pollen viability, a cultivar (Yangmai 18) was subjected to heat stress for 15 to 19 days after flowering in a field warming-shed. In this study, we compared the effects of SA pretreatment on antioxidant physiological characteristics of wheat at different stages. After heat stress, malondialdehyde (MDA) and hydrogen peroxide (H2O2) content increased, superoxide anion ( ) was accelerated, lipid peroxidation was observed, and the wheat flag leaf was damaged. The results showed that exogenous SA could alleviate heat-induced membrane damage by reducing electrolyte leakage (EL) and malondialdehyde (MDA) content, and regulate the activities of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD). Impacts on flag leaves including the increase in the activities of stress resistance indicators, phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) indicates that exogenous SA enhances tolerance to high temperature environments and are beneficial to increase the photosynthetic membrane integrity, thereby delaying the senescence rate of flag leaves. After spraying exogenous SA in different periods, salicylic acid at the flowering stage treatment had the best effect on alleviating high temperature stress damage compared with other treatments. The results revealed that exogenous SA application of enhanced exogenous spraying of SA can alleviate the damage of high temperature stress on wheat flag leaves during the filling stage by increasing the activities of antioxidant enzymes and stress-resistant enzymes in wheat flag leaves. The results of this study provide a new technical approach to reduce the harmful effect of high temperatures on wheat production. |
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| Keywords: | wheat salicylic acid filling stage flag leaf oxidation characteristics |
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