| 国槐树枝不同溶剂提取物对植物病原菌的抑菌活性 |
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| 引用本文： | 张煜,李运盛,成军,江幸福,刘悦秋,吴昱颖.国槐树枝不同溶剂提取物对植物病原菌的抑菌活性[J].中国农学通报,2022,38(33):68-73. |
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| 作者姓名： | 张煜 李运盛 成军 江幸福 刘悦秋 吴昱颖 |
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| 作者单位： | 1.北京农学院园林学院，北京 102206;2.北京农学院生物与资源环境学院，北京 102206;3.植物病虫害生物学国家重点实验室，中国农业科学院植物保护研究所，北京 100193 |
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| 基金项目： | 国家重点研发计划“主要农林废弃物提取加工与功效评价标准研究”(2017YFF0207800);北京市教委科技计划“北京市苗圃节水技术体系研究”(KM201810020012);北京农学院本科生科研训练项目“国槐树枝活性成分提取分离”(KX2020019) |
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| 摘 要： | 国槐(Sophora japonica L.)是中国重要的园林植物和药用植物。本研究旨在挖掘国槐在抑制植物病原菌方面的功能。采用生长速率法测定国槐树枝中乙醇提取物和不同极性溶剂（石油醚、乙酸乙酯、正丁醇与水）萃取物对4种植物病原菌的抑制活性并运用气质联用技术分析乙酸乙酯萃取物的化学成分。结果表明，国槐树枝提取物及其萃取物均对4种植物病原菌有一定的抑制作用且差异显著。其中乙酸乙酯萃取物的抑菌效果最好，它对胶孢炭疽菌(Colletotrichum gloeosporioides)的毒性最高，48 h的EC50为2.58 mg/mL，其次为Ectophoma multirostrata、灰葡萄孢菌(Botrytis cinerea)和臭镰刀菌(Fusarium foetens)。从乙酸乙酯萃取物检测出其主要化学成分是脂肪族和萜类化合物，含量最高的是五氟丙酸十八烷基酯(14.77%)。国槐树枝中起抑菌作用的活性物质主要集中于乙酸乙酯相，其次为正丁醇相、水相与石油醚相。
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| 关 键 词： | 国槐树枝 提取物 抑菌活性 化学成分 |
| 收稿时间： | 2021-11-24 |
Antifungal Activities of the Extracts from Branches of Sophora japonica by Different Solvents |
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| ZHANG Yu,LI Yunsheng,CHENG Jun,JIANG Xingfu,LIU Yueqiu,WU Yuying.Antifungal Activities of the Extracts from Branches of Sophora japonica by Different Solvents[J].Chinese Agricultural Science Bulletin,2022,38(33):68-73. |
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| Authors: | ZHANG Yu LI Yunsheng CHENG Jun JIANG Xingfu LIU Yueqiu WU Yuying |
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| Institution: | 1.School of Landscape Architecture, Beijing University of Agriculture, Beijing 102206;2.School of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206;3.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193 |
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| Abstract: | The Chinese scholar tree (Sophora japonica L.) is an important garden plant and medicinal plant in China. The study aims to explore the function of Sophora japonica in inhibiting plant pathogens. The inhibitory activities of branch extracts by different polar solvents (ethanol, petroleum ether, ethyl acetate, n-butanol and water) against four plant pathogens were determined by using growth rate method, and the chemical components of ethyl acetate extract were analyzed by GC-MS. The results showed that the extracts of Sophora japonica branches by different solvents had significantly different inhibitory effects against four plant pathogens. Ethyl acetate extract had the best antifungal effect, and its toxicity to Colletotrichum gloeosporioides was the highest, with an EC50 of 2.58 mg/mL at 48 h, followed by its toxicity to Ectohoma multirostrata, Botrytis cinerea and Fusarium foetens. The main chemical components of ethyl acetate extract were aliphatic and terpenoids, and the highest content was octadecyl pentafluoropropionate (14.77%). The antifungal active substances in the branches of Sophora japonica were mainly concentrated in the ethyl acetate phase, followed by n-butanol phase, water phase, and petroleum ether phase. |
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| Keywords: | Sophora japonica branches extracts antifungal activity chemical component |
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