| 基于UPLC-MS/MS分析不同生态型香蕉品种青果皮的主要酚酸成分 |
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| 引用本文： | 张晶,刘立娜,杨宝明,王永芬,何平,徐胜涛,尹可锁,李舒,白亭亭,李永平,李迅东,郑泗军.基于UPLC-MS/MS分析不同生态型香蕉品种青果皮的主要酚酸成分[J].中国农学通报,2022,38(28):129-135. |
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| 作者姓名： | 张晶 刘立娜 杨宝明 王永芬 何平 徐胜涛 尹可锁 李舒 白亭亭 李永平 李迅东 郑泗军 |
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| 作者单位： | 1.云南省农业跨境有害生物绿色防控重点实验室/云南省农业科学院农业环境资源研究所,昆明 650205;2.江南大学环境与土木工程学院,江苏无锡 214122;3.云南省农业科学院热带亚热带经济作物研究所,云南保山 678000;4.国际生物多样性中心,昆明 650205 |
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| 基金项目： | 国家自然科学基金项目“解析不同生态型香蕉对枯萎病的抗性机制”(NSFC32161143001);云南省万人计划“云岭学者”项目“香蕉枯萎病抗性与病原菌互作的分子机理与研究”(YNWR-YLXZ-2018-018);中国农业农村部国家香蕉产业技术体系专项“国家香蕉产业技术体系红河综合试验站”(CARS-31) |
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| 摘 要： | 为充分挖掘和利用不同生态型香蕉品种青果皮的功能活性物质,建立超高效液相色谱-电喷雾电离串联质谱联用法(UPLC-MS/MS)对各香蕉品种青果皮的主要酚酸类化合物进行定量分析。使用Agilent ZORBAX Extend-C18色谱柱(2.1 mm×100 mm,3.5 μm),甲醇(A)-0.2%甲酸水溶液(B)进行梯度洗脱,流速0.3 mL/min,进样量5 μL,柱温35℃;在负离子扫描模式、多反应监测模式下,采用电喷雾离子化源进行检测。结果表明,7种酚酸类化合物（没食子酸、奎尼酸、原儿茶酸、儿茶素、绿原酸、阿魏酸和芦丁）分离效果良好,质量分数分别在1~200、1~2000、1~200、1~200、1~200、2~200、1~500 μg/L范围内表现出良好的线性相关关系(r2>0.9992),平均加标回收率(n=6)84.37%~92.17%,RSD≤10.96%。试验所建立的UPLC-MS/MS法具有准确、灵敏、快速、重复性好的特点,可用于同时测定香蕉青果皮中7种酚酸化合物的含量。不同生态型香蕉品种青果皮中主要酚酸含量差异显著,非鲜食蕉的青果皮酚酸含量显著高于鲜食蕉,尤其以非鲜食蕉中‘Pahang’青果皮酚酸含量最高,达到2.12 mg/g。
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| 关 键 词： | 超高效液相色谱-串联质谱 生态型香蕉 青果皮 酚酸 |
| 收稿时间： | 2021-10-13 |
Analysis of Main Phenolic Acids in Green Peel of Different Ecological Banana Varieties Based on UPLC-MS/MS |
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| ZHANG Jing,LIU Lina,YANG Baoming,WANG Yongfen,HE Ping,XU Shengtao,YIN Kesuo,LI Shu,BAI Tingting,LI Yongping,LI Xundong,ZHENG Sijun.Analysis of Main Phenolic Acids in Green Peel of Different Ecological Banana Varieties Based on UPLC-MS/MS[J].Chinese Agricultural Science Bulletin,2022,38(28):129-135. |
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| Authors: | ZHANG Jing LIU Lina YANG Baoming WANG Yongfen HE Ping XU Shengtao YIN Kesuo LI Shu BAI Tingting LI Yongping LI Xundong ZHENG Sijun |
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| Institution: | 1.Yunnan Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests/ Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205;2.School of Environmental and Civil Engineering, Jiangnan University, Wuxi, Jiangsu 214122;3.Institute of Tropical and Subtropical Industry Crops, Yunnan Academy of Agricultural Sciences, Baoshan, Yunnan 678000;4.Bioversity International, Kunming 650205 |
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| Abstract: | In order to fully explore and utilize the functional active substances of the green peel of different ecological banana varieties, an ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-MS/MS) method was established to quantitatively analyze main phenolic acids in the green peel of various banana varieties. Agilent ZORBAX Extend-C18 column (2.1 mm×100 mm, 3.5 μm) was used, gradient elution was conducted with methanol (A)-0.2% formic acid aqueous solution (B), flow rate of 0.3 mL/min, injection volume of 5 μL, column temperature of 35℃. Under the negative ion scanning mode and the multi-reaction monitoring mode, the electrospray ionization source was detected. The separation effect of seven phenolic acids (gallic acid, quinic acid, protocatechuic acid, catechin, chlorogenic acid, ferulic acid and rutin) was good, and the mass scores showed good linear correlation in the range of 1-200, 1-2000, 1-200, 1-200, 1-200, 2-200, and 1-500 μg/L (r2>0.9992), respectively, the average recovery (n=6) was 84.37%-92.17%, and RSD≤10.96%. The established UPLC-MS/MS method had the characteristics of accuracy, sensitivity, rapidity and good repeatability, and could be used for the simultaneous determination of the content of the seven phenolic acids in green banana peel. The content of main phenolic acids in the green peel of different ecological banana varieties was significantly different. The phenolic acid content in green peel of non-dessert bananas was significantly higher than that of dessert bananas, and the phenolic acid content in green peel of non-dessert banana ‘Pahang’ was the highest, which was 2.12 mg/g. |
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| Keywords: | ultra performance liquid chromatography-tandem mass spectrometry ecological bananas green peel phenolic acid |
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